Mechanism of Salidroside in Improving Glucose and Lipid Metabolism in Liver of Diabetic Rats
10.13422/j.cnki.syfjx.20182124
- VernacularTitle: 红景天苷改善糖尿病大鼠肝脏糖脂水平的作用机制
- Author:
Dan HOU
1
;
Guang-yuan XU
2
;
Zhuo ZHANG
1
;
Tong-hua LIU
1
Author Information
1. Institute of Healthcultivation, Beijing University of Chinese Medicine, Beijing 100029, China
2. Fu Xing Hospital, Capital Medical University, Beijing 100045, China
- Publication Type:Research Article
- Keywords:
salidroside;
oxidative stress;
diabetes;
PKR-like ER kinase/NF-E2-relatedfactor2/heme oxidase-1(HO-1)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):130-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the mechanism of salidroside(SAL) in improving glucose and lipid metabolism in liver of diabetic rats. Method: According to blood glucose, 24 male ZDF(fa/fa) rats of 6-7 week old were randomly divided into model group, SAL-low group (0.05 g·kg-1), SAL-high group (0.1 g·kg-1), 8 rats in each group. Another 8 ZDF (fa/+) rats were selected as normal group. All the rats were administered continuously for 6 weeks. After experiment, fasting blood glucose(FBG), triglyceride(TG), total cholesterol(TC), free fatty acids(FFA), superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), fasting insulin(Fins), and homeostasis model assessment insulin resistance(HOMA-IR) were measured. Western blot was used to detect phosphorylated PKR-like ER kinase(p-PERK), NF-E2-relatedfactor2(Nrf2), heme oxidase-1(HO-1) protein expression levels in liver. Result: As compared with normal group, FBG, Fins, HOMA-IR, TC, TG, FFA, and MDA were increased significantly in model group (P <0.05, P<0.01), while SOD, CAT levels were significantly decreased (P<0.05, P<0.01); the protein expression levels of p-PERK, Nrf2, and HO-1 in control group were significantly decreased in model group(P<0.05, P<0.01). As compared with model group, FBG, Fins, HOMA-IR, TC, TG, FFA, and MDA levels were decreased significantly (P<0.05, P<0.01), while SOD and CAT levels were significantly increased in SAL low and high groups (P<0.05, P<0.01); and the protein expression levels of p-PERK, Nrf2, and HO-1 in SAL group were significantly increased(P<0.05,P<0.01). Conclusion: The Salidroside can improve metabolism of glucose and lipid, IR in diabetic rats, and the mechanism may be associated with inhabiting oxidative stress through the up-regulation of p-PERK, Nrf2, and HO-1 protein expression.
