Tanshinone IIA inhibits apoptosis, invasion and migration of esophageal cancer EC9706 and KYSE70 cells by regulating epithelial-mesenchymal transition
10.3872/j.issn.1007-385x.2018.12.006
- VernacularTitle:丹参酮IIA通过调控上皮间质转化抑制食管癌EC9706和KYSE70细胞 的凋亡、侵袭和迁移
- Author:
ZHOU Pengfei
1
;
LI Juan
2
Author Information
1. Department of Thoracic Oncology, Cancer Hospital of Meishan
2. Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Chengdu Medical College
- Publication Type:Journal Article
- Keywords:
tanshinone IIA;
esophageal cancer;
EC9706 cell;
KYSE70 cell;
epithelial-mesenchymal transition(EMT)；apoptosis;
invasion;
migration
- From:
Chinese Journal of Cancer Biotherapy
2018;25(12):1244-1250
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of tanshinone IIA on the invasion and migration of esophageal cancer EC9706 and KYSE70 cells, and to explore the underlying mechanism. Methods: Esophageal cancer cells (EC9706 and KYSE70) were divided into 4 groups: control group, tanshinone IIA groups (2, 4, 6 μg/ml). Cell prliferation viability was measured by CCK-8; Apoptosis was detected by flow cytometry; Invasion was tested by Transwell assay; And migration was measured by Scratch assay. The mRNA and protein levels of E-cadherin, Snail-2, Vimentin and N-cadherin were tested by quantitative Real-time reverse transcription PCR (qRT-PCR) and Western blotting, respectively. Results: Tanshinone IIAat concentrations less than 6 μg/ml did not affect the cell viability of esophageal cancer EC9706 and KYSE70 cells. The apoptosis in tanshinone IIA (4, 6 μg/ml) groups was significantly higher than that in control group ( P < 0.01). The number of invasive cells per field and wound-healing rate in tanshinone IIA(2, 4, 6 μg/ml) groups were significantly lower than those in control group (all P <0.01). Moreover, the cell morphology was transformed from a spindle-shaped mesenchymal form into epithelial morphology after tanshinone IIA treatment. Compared with control group, the expression of E-cadherin in tanshinone IIA groups (2, 4, 6 μg/ml) was significantly up-regulated while the expressions of Snail-2, Vimentin and N-cadherin were significantly down-regulated (all P <0.01). Conclusion: Tanshinone IIApromotes apoptosis and attenuates the invasion and migration of esophageal cancer cells by inhibiting the epithelial-mesenchymal transition.
- Full text:20181206.pdf
