Effect of long-chain non-coding RNA TUG1 on radiosensitivity of human cervical cancer XB1702 cells by adsorption of miR-145
10.3760/cma.j.issn.1004-4221.2019.12.013
- VernacularTitle: 长链非编码RNA TUG1通过吸附miR-145对XB1702细胞放射敏感性影响
- Author:
Xiuling LIU
1
;
Sen WANG
2
;
Zhihong WANG
3
;
Jing LI
4
;
Xinyu CHEN
5
;
Quanqin HE
1
Author Information
1. Department of Gynaecology, Zhumadian Central Hospital, Zhumadian 463000, China
2. Department of Radiotherapy, Zhumadian Central Hospital, Zhumadian 463000, China
3. Department of Obstetrics and Gynecology, Second Affiliated Hospital of Zhengzhou University, 450000 Zhengzhou, China
4. Department of Obstetrics and Gynecology, Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China
5. Department of Oncology, Zhumadian Center Hospital, Zhumadian 463000, China
- Publication Type:Journal Article
- Keywords:
TUG1 gene;
miR-145 gene;
Radiosensitivity;
Cervical cancer cell line
- From:
Chinese Journal of Radiation Oncology
2019;28(12):939-941
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of long-chain non-coding RNA TUG1 on the radiosensitivity of cervical cancer cells and explore its underlying mechanism.
Methods:The expression of TUG1 and miR-145 in cervical cancer cells XB1702 and normal endometrial stromal cells (ESCs) was detected by qRT-PCR. The transfected si-NC, transfected si-TUG1, transfected si-NC combined with irradiation, transfected si-TUG1 combined with irradiation, si-TUG1 and anti-miR-NC co-transfected and, si-TUG1 and anti-miR-145 co-transfected groups were established, which were transfected into XB1702 cells by liposome method. The survival fraction of each group was detected by colony formation assay. The cell apoptosis of each group was detected by flow cytometry. The fluorescence activity of each group was assessed by dual luciferase reporter gene assay.
Results:Compared with the normal ESCs, the expression of TUG1 was significantly up-regulated, whereas that of miR-145 was significantly down-regulated in the cervical cancer cells XB1702. Silencing TUG1 significantly increased the survival fraction of XB1702 cells, promoted cell apoptosis and enhanced the radiosensitivity of irradiation to XB1702 cells. TUG1 could target and regulate the expression of miR-145. Suppressing miR-145 reversed the silencing effect of TUG1 on inhibiting proliferation, accelerating apoptosis promotion and enhancing sensitization of XB1702 cells.
Conclusions:Silencing long-chain non-coding RNA TUG1 can enhance the radiosensitivity of cervical cancer cells. The mechanism may be related to targeting miR-145, which will provide a target for radiotherapy of cervical cancer.
