The effect and mechanism of transient receptor potential M2 in antigen-induced arthritis mice
10.3760/cma.j.issn.0578-1426.2019.12.009
- VernacularTitle: 瞬时受体电位M2对抗原诱导关节炎小鼠的作用及机制研究初探
- Author:
Mingyao LI
1
;
Yi ZHAO
2
;
Yubin LUO
2
;
Yanhong LI
2
;
Yi LIU
3
Author Information
1. Department of Rheumatology and Immunology, Subsidiary Hospital, Southwestern Medical University, Luzhou, Sichuan Province 646000, China
2. Department of Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu 610041, China
3. Department of Rheumatology and Immunology, Subsidiary Hospital, Southwestern Medical University, Luzhou, Sichuan Province 646000, China; Department of Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu 610041, China
- Publication Type:Case Reports
- Keywords:
Arthritis, rheumatoid;
Antigen-induced arthritis;
Transient receptor potential M2;
Innate immunecells
- From:
Chinese Journal of Internal Medicine
2019;58(12):911-914
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to explore the role and mechanism of transient receptor potential M2 (TRPM2) in antigen-induced arthritis (AIA) mice. Twelve C57BL/6 mice and 12 TRPM2 knockout mice were divided into 4 groups, includingwild type control group, wild type AIA group, TRPM2 knockout control group and TRPM2 knockout AIA group, with 6 mice in each group. Methylated bovine serum albumin (mBSA) was used to establish AIA mouse model. The degree of joint swelling and inflammatory cell infiltration were recorded, as well as synovial hyperplasia of the knee joints. Real-time quantitative polymerase chain reaction (PCR) was used to detect the expression of interleukin (IL)-6, IL-8, chemokine ligand 6 (CXCL-6) and tumor necrosis factor alpha (TNFα) mRNA in synovial cells of knee joints. The results showed that compared with the wild-type AIA group, the TRPM2 knockout AIA group had more significant synovial proliferation and inflammatory cell infiltration in the synovial tissue.The neutrophil and macrophage counts rather than monocytes in the knee joints of TRPM2 knockout AIA group were higher than those in wild-type AIA mice. The expression of IL-6, IL-8 and CXCL-6 mRNA were significantly increased in the knock out mice. In summary, TRPM2 may inhibit inflammatory cytokines such as IL-6 and IL-8 in knee joints of AIA mice by reducing the infiltration of neutrophils and macrophages, the refore alleviates the manifestations of knee arthritis.
