Change and relationship between Gli1 and β-catenin on rats′ bone formation with chronic fluorosis
10.3760/cma.j.issn.0529-5807.2020.02.012
- VernacularTitle: 慢性氟中毒大鼠骨组织Gli1、β-catenin表达变化及其与骨形成增强间的相关性分析
- Author:
Chaonan DENG
1
;
Ying ZHANG
2
;
Lin XU
2
;
Lina ZHAO
1
;
Yan LINGHU
3
;
Yanni YU
1
Author Information
1. Department of Pathology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China; Department of Pathology, Guizhou Medical University, Guiyang 550004, China
2. Department of Pathology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China; Maternal and Child Care Hospital of Guiyang, Guiyang 550004, China
3. Department of Pathology, Guizhou Medical University, Guiyang 550004, China
- Publication Type:Journal Article
- Keywords:
Rats;
Bone and bones;
Fluorosis
- From:
Chinese Journal of Pathology
2020;49(2):168-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the change and association of glioma-associated oncogene homolog 1 (Gli1) and β-catenin on bone formation in rats with chronic fluorosis which were inhibited by cyclopamine (Cycl).
Methods:Forty-eight Sprague-Dawley rats were evenly divided to four groups, including control, F, F+Cycl and F+DMSO groups. The control group were fed with tap water (NaF<1 ppm). The F, F+Cycl and F+DMSO groups were exposed to NaF (50 ppm) in drinking water as the chronic fluorosis model. Then the rats in F+Cycl or F+DMSO groups were injected by Cycl or DMSO after 6 months, respectively. Urine fluoride concentration was detected using fluorine ion selective electrode. The enzyme-linked immunosorbent assay (ELISA) was used to detect bone alkaline phosphatase (BALP). Bone tissues were stained with hematoxylin-eosin. The mRNA and protein expression of Gli1 and β-catenin in bone tissue were detected using real-time PCR, immunohistochemistry and Western blot.
Results:Compared with the controls, the urine fluoride concentration and the width and volume of bone trabeculae were increased in the F, F+Cycl and F+DMSO groups, but no statistical difference among the 3 fluorosis groups. The concentration of BALP was increased in the F group and decreased in F+Cycl group (P<0.05). The expression of Gli1 and β-catenin mRNA and protein was higher in the F and F+Cycl groups than controls, but lower in the F+Cycl group than in the F group. There was positive correlation between the expression of Gli1 and β-catenin (r=0.476, P<0.05). The expression of Gli1 and β-catenin was also associated with BALP concentration and volume of bone trabeculae, respectively (r1=0.457, r2=0.466, r3=0.581, r4=0.554, respectively, P<0.05 for all).
Conclusions:The expression of Gli1 can be inhibited by Cycl. It may be involved in the bone formation of rats with chronic fluorosis. It may also affect the expression of β-catenin, which is an osteogenesis factor.
