Effect of different compatibility ratios of Centella asiatica-Rhubarb on the dissolution rate of effective components of Centella asiatica
10.3760/cma.j.issn.1673-4246.2020.01.012
- VernacularTitle: 积雪草-大黄不同配伍比例对积雪草有效成分溶出率的影响
- Author:
Yi HAN
1
;
Chundi LIU
1
;
Hui YU
1
;
Jie LI
1
Author Information
1. Pharmacy Department of Zhangjiagang Hospital Affiliated to Nanjing University of Traditional Chinese Medicine, Zhangjiagang 215600, China
- Publication Type:Journal Article
- Keywords:
Centellae herba;
Rhei radix et rhizoma;
Compatibility (TCD);
Madecassoside;
Asiaticoside;
Assay (TCD)
- From:
International Journal of Traditional Chinese Medicine
2020;42(1):58-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of different compatibility ratios of Centella asiatica- Rhubarb on the dissolution rates of madecassoside and asiaticoside.
Methods:By using the HPLC method to analyze the content of madecassoside and asiaticoside in the Centella asiatica extract solution as well as Centella asiatica - Rhubarb extract solution with 6 different kinds of compatibility ratios. The tests were carried out by Thermo C18 (250 mm×4.6 mm, 5 μm) by isocratic elution with acetonitrile and 2 mmol/L of beta-cyclodextrin solution as mobile phase at a flow rate of 1 ml/min, the column temperature was 30 ℃ and the detection wavelength was 205 nm.
Results:Compared with the Centella asiatica extract solution, the dissolution rate of madecassoside decreased significantly with the increase of the proportion of rhubarb. The combination of Centella asiatica and Rhubarb had little effect on the content of asiaticoside.
Conclusions:Considering the clinical application and efficacy of Centella asiatica, the optimal ratio of Centella asiatica-Rhubarb is 10:1 and 15:1.
