Chemical Constituents of Lipid-soluble Parts of Tinospora sinensis
10.13422/j.cnki.syfjx.20190216
- VernacularTitle: 宽筋藤脂溶性部位的化学成分分离鉴定
- Author:
Xiao-fang ZHU
1
;
Ya-juan MA
1
;
Wen-ting BAI
1
;
Li-ping HUANG
1
;
Yu-hui PING
1
;
Yi-hui XIE
1
Author Information
1. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
Tinospora sinensis;
rhizome;
liposoluble components;
n-dodecanol;
n-hexacosanol
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(2):163-168
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate the chemical constituents from lipid-soluble parts of Tibetan Tinospora sinensis and identify the structure of the monomer compounds. Method:Eighty kg of dried rhizomes of T. sinensis were soaked by 80% ethanol (5 times of the dose of crude drug) for 1 hour, boiled under reflux for 1 hour,then and filtered. The above steps were repeated. The two extracts were combined,and the solvent was recovered to obtain a total extract. The obtained extract was extracted with a conventional solvent,and the solution was recovered to obtain petroleum ether extract,methylene chloride extract,ethyl acetate extract,n-butanol extract and water extract. Methylene chloride extract was isolated and purified by silicagel column chromatography,semi-preparative liquid chromatography and SephadexLH-20 chromatography; the chemical structure was identified on the basis of ESI-MS,nuclear magnetic resonance (1H-NMR and 13 C-NMR) spectroscopy data and literatures. Result:Fifteen compounds were isolated and identified respectively as n-dodecanol (1),n-hexacosanol (2),palmitic acid (3),dibutyl phthalate (4),vanillic acid (5),vanillin (6),apocynin (7),tinocordifolioside (8),medioresinol (9),isolariciresinol (10),aurantiamide (11),aurantiamide acetate (12),berberine (13),daucosterol (14),β-sitosterol (15). Conclusion:Except for 3,4,6,14,15,the other 10 compounds were isolated from the plant for the first time.
