Preparation,Characterization and in Vitro and in Vivo Absorption Characteristics of Daidzein Nanosuspension Capsules
10.13422/j.cnki.syfjx.20182301
- VernacularTitle: 黄豆苷元纳米混悬剂胶囊的制备、表征及其体内外吸收特性考察
- Author:
Xiao-ming YANG
1
;
Zun HUANG
1
;
Jun ZHANG
1
;
Tian-tian ZUO
1
;
Qi SHEN
1
Author Information
1. School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China
- Publication Type:Research Article
- Keywords:
daidzein;
nanosuspensions;
capsules;
high pressure homogenization technology;
in vitro intestinal absorption;
pharmacokinetics;
bioavailability
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(2):40-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare daidzein nanosuspension capsules,and to investigate intestinal absorption and oral bioavailability by comparing with commercial daidzein capsules. Method:Daidzein nanosuspensions were prepared by precipitation method combined with high pressure homogenization,orthogonal design method was utilized to optimize its formulation.Daidzein nanosuspensions was characterized by X-ray powder diffraction(XRPD),Fourier transform infrared spectroscopy(FT-IR),transmission electron microscope(TEM),and indexes including mean particle size,polydispersity index(PDI),and Zeta potential.Intestinal absorption study was carried out to compare the accumulative permeated amount of daidzein from daidzein nanosuspensions and commercial daidzein capsules.Biodistribution of daidzein in gastrointestinal tract was investigated,and oral bioavailability was examined through pharmacokinetic study by HPLC. Result:The in vitro small intestinal absorption enhancement ratio of daidzein nanosuspension capsules was approximately 2.49-fold higher than that of commercial capsules(P<0.01).Biodistribution evaluation in gastrointestinal tract verified the bioadhesion of the nanosuspensions.Pharmacokinetic study calculated by DAS 2.0 revealed that the relative bioavailability of daidzein nanosuspension capsules was 294%. Conclusion:Daidzein nanosuspensions prepared by combined method can be applied to the production of capsules,which is beneficial to increase the absorption of drug in small intestine and improve its bioavailability after oral administration.
