Effect of Processing with Vinegar on Hepatorenal Toxicity of Dichloromethane Site of Genkwa Flos
10.13422/j.cnki.syfjx.20182203
- VernacularTitle: 醋炙降低芫花二氯甲烷部位对大鼠肝肾细胞毒性的分析
- Author:
Qin-rong ZHOU
1
;
Pan-pan SUN
1
;
Lin-yan XIAO
1
;
Qi-nan LIU
1
;
Li ZHANG
1
Author Information
1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China
- Publication Type:Research Article
- Keywords:
Genkwa Flos;
processing with vinegar;
NRK;
BRL;
reducing toxicity;
hepatorenal cells;
oxidative damage
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(2):35-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To campare the hepatotoxicity on BRL and nephrotoxicity on NRK caused by dichloromethane site of Genkwa Flos before and after being processed with vinegar. Method:BRL of normal hepatocytes and NRK of normal renal cells in rats were selected as the subjects.Thiazolyl blue tetrazolium bromide method(MTT) was adopted to evaluate the effect of dichloromethane sites of raw and vinegar-processed products on cell activity of NRK and BRL.The levels or contents of aspartate aminotransferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(ALP),glutathione(GSH),lactate dehydrogenase(LDH),blood urea nitrogen(BUN) were determined in cell culture supernatant and splitting supernatant for evaluation of their oxidative damage effect. Result:Compared with the blank group,dichloromethane site of raw products could obviously inhibit the cell activity of NRK and BRL,and increase the levels of AST,ALT,ALP and LDH(P<0.05,P<0.01) in BRL and decrease the content of GSH;meanwhile,it could obviously increase the levels of LDH and BUN(P<0.05,P<0.01) and decrease the content of GSH in NRK.Compared with corresponding dose group of raw products,vinegar-processed products could improve cell activity of NRK and BRL,and decrease the levels of AST,ALT,ALP and LDH in BRL and increase the content of GSH;also,it decreased the levels of LDH and BUN and increased the content of GSH in NRK. Conclusion:Processing with vinegar can attenuate the hepatotoxicity and nephrotoxicity on NRK and BRL caused by dichloromethane site of Genkwa Flos,it can improve hepatic and renal function and antioxidant capacity.
