UPLC-Q-TOF-MS/MS Analysis of Brain and Blood Absorption Components of Buyang Huanwu Tang After Intragastric Administration
10.13422/j.cnki.syfjx.20182309
- VernacularTitle: 补阳还五汤吸收入脑及入血成分的UPLC-Q-TOF-MS/MS分析
- Author:
Xiao SHEN
1
;
Hua-zhu ZHENG
1
;
Ying-jiao MENG
1
;
Xiang-li YAN
1
;
Sheng-xin WANG
1
;
Ai-ming YU
1
;
Li-sheng WANG
1
Author Information
1. School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
- Publication Type:Research Article
- Keywords:
Buyang Huanwu Tang;
calycosin-7-glucoside;
albiflorin;
cerebral homogenate;
formononetin-7-O-β-D-glucoside-6″-O-acetyl;
plasma;
prototype components
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(2):8-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze and identify the brain and blood absorption components of rats after intragastric administration of Buyang Huanwu Tang(BYHWT). Method:The brain tissue,plasma of normal rats and the cerebral ischemia-reperfusion rats were analyzed by UPLC-Q-TOF-MS/MS.The prototype components in BYHWT were identified according to retention time,accurate relative molecular weight,primary and secondary mass spectrometry data. Result:After the administration of BYHWT,five compounds were found to enter the normal brain tissue through the blood-brain barrier and identified as calycosin-7-glucoside,albiflorin,formononetin-7-O-β-D-glucoside-6″-O-acetyl,safflower yellow A and astragaloside A;two compounds penetrated the blood-brain barrier and entered modeling brain tissue,and they were identified as calycosin-7-glucoside and formononetin-7-O-β-D-glucoside-6″-O-acetyl;seven compounds entered normal plasma and were identified as calycosin-7-glucoside,albiflorin,hydroxysafflor yellow A,et al;three compounds entered model plasma and identified as calycosin-7-O-β-D-glucoside-6″-O-acetyl,6″-O-acetyl-(6αR,11αR)-9,10-dimetho-xypterocarpan-3-O-β-D-glucoside and formononetin-7-O-β-D-glucoside-6″-O-acetyl. Conclusion:BYHWT has different pharmacological material basis in normal and cerebral ischemia-reperfusion rats.
