Development of Novel Microsatellite Markers and Characteristic Analysis for Sinowilsonia henryi
10.13422/j.cnki.syfjx.20190320
- VernacularTitle: 山白树微卫星特征分析及分子标记开发
- Author:
Jia WANG
1
;
Tian-hua ZHOU
1
Author Information
1. School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China
- Publication Type:Research Article
- Keywords:
Sinowilsonia henryi;
microsatellite;
characteristics analysis;
molecular marker;
genome;
polymorphism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(3):143-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the microsatellite characteristics and develop microsatellite markers for Sinowilsonia henryi, a rare and endangered plant endemic to China.Method: The Illumina second generation sequencing technology was used to establish the genome library and the microsatellite library of S. henryi. The compositions of the microsatellite were analyzed,and the primers of S. henryi were designed. Its polymorphism was analyzed by the amplification and detection of 5 S. henryi populations.Result: A total of 38,942,and 660 matched sequences of 100 bp were returned by the second generation sequencing. Among them,200,386 splice sequences were obtained by mass pruning and splicing; the number of sequences with length greater than 335 bp was 7 614, and 694 microsatellite loci were detected in the 7 614 sequences,in which the single nucleotide repeats were most and the number of A/T repeated in the single nucleotide repeats was highest. Dinucleotide showed the greatest length variation; the number of AG/CT repeats was highest, and the variation in repeat length was positively correlated with microsatellite abundance. The primers were designed for 36 S. henryi microsatellite sequences with high repetition. By PCR amplification and polyacrylamide gel electrophoresis,20 pairs of primers showed rich polymorphism and clear bands. The number of alleles (NA) ranged from 3 to 6,with average of 4;the polymorphism information content (PIC) ranged from 0.535 5 to 0.754 0,with average of 0.615 5.The population genetic analysis of 5 S. henryi populations showed that the genetic diversity of the species was high (h=0.697 5,I=1.436 8,HE=0.702 2),and the population genetic differentiation was significant (Fst=0.374).Conclusion: The population genetic analysis of S. henryi also showed that the primers developed by this study had a high usability. In this study,the primers of microsatellite markers of S. henryi were established,laying the foundation for the molecular genetics of S. henryi.
