Effect of Xiao Chaihutang and Wulingsan on Protection of Residual Renal Function in Patients with Maintenance Peritoneal Dialysis and Effect on Peritoneal Fibrosis and Micro-inflammation
10.13422/j.cnki.syfjx.20182425
- VernacularTitle: 小柴胡汤合五苓散对维持性腹膜透析患者残余肾功能的保护及对腹膜纤维化、微炎症状态的影响
- Author:
Jin ZHONG
1
;
Ling-yun ZHONG
1
Author Information
1. Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, China
- Publication Type:Research Article
- Keywords:
Xiao Chaihutang;
Wulingsan;
patients with end-stage renal disease;
residual renal function;
micro-inflammatory state
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(3):114-119
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the protective effect of Xiao Chaihutang and Wulingsan on residual renal function in patients with maintenance peritoneal dialysis (PD) and investigate its effect on peritoneal fibrosis and microinflammation.Method: The 65 patients with PD who were admitted to our hospital from June 2016 to June 2017 were enrolled in the study, and divided into control group (32 cases) and study group (33 cases) according to the random number table. The control group received routine treatment. The study group received routine treatment+Xiao Chaihutang and Wulingsan. The fasting venous blood was taken before treatment and 3 months after treatment to measure serum creatinine (SCr) and urea nitrogen (BUN). Urine was collected; 24 hour urine volume was recorded; 24 h urine protein (24 h UP) was measured by colorimetry; glomerular filtration rate (eGFR) was calculated; residual renal function (RRF) was expressed with residual renal creatinine clearance. Inflammatory factors were detected by using chemiluminescence, including interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) levels. Serum Janus Kinase (JAK) 2 as well as signal transducer and activator of transcription (STAT) 3 levels were determined by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). E-cadherin and α-smooth muscle actin (α-SMA) levels were determined by Western blot.Result: Before treatment, there was no significant difference in the residual renal function between two groups. After treatment, the residual renal function of the study group was significantly better than that of control group (P<0.05). After treatment, 24 h UP of study group was lower than that of control group, and the daily average urine volume was higher than that of control group (P<0.05). After treatment, the levels of IL-6, IL-8 and TNF-α in study group were lower than those in control group (P<0.05); the levels of JAK2 and STAT3 in study group were significantly lower than those in control group (P<0.01). The expression of E-cadherin protein in the dialysate of control group was significantly decreased after treatment (P<0.01), and the expression level of α-SMA protein was significantly increased after treatment (P<0.01); however, study group had no significant changes in the expression of E-cadherin and α-SMA protein after treatment.Conclusion: Xiao Chaihutang and Wulingsan can protect the residual renal function of PD patients, and the mechanism may be related to the improvement of peritoneal fibrosis and the reduction of micro-inflammation of the body, showing a high application value.
