Safety Evaluation of Over-ground Parts of Glycyrrhiza uralensis
10.13422/j.cnki.syfjx.20190218
- VernacularTitle: 甘草地上部分的安全性评价
- Author:
Zi-han ZHAO
1
;
Lin ZHANG
1
;
Liu YANG
1
;
Jie CUI
2
;
Jun-ling HOU
1
;
Wen-quan WANG
2
;
Da-yan LI
1
Author Information
1. School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China
2. Institute of Medicinal Plant Development, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100193, China
- Publication Type:Research Article
- Keywords:
over-ground parts of Glycyrrhiza uralensis;
safety evaluation;
acute toxicity;
genetic toxicity;
liver and kidney toxicity
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(3):74-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the toxicological safety of over-ground parts of Glycyrrhiza uralensis, in order to provide basis for the rational utilization of over-ground parts of Glycyrrhizae Radix et Rhizoma recourses.Method: Mice acute oral toxicity test, micronucleus test of mice bone marrow, mice sperm shape abnormality test and toxicological test based on chronic nonbacterial prostatitis model were carried out.Result: Maximal tolerable dose(MTD) of over-ground parts of G.uralensis water extract (WE) and alcohol extract (AE) were 96,128 g·kg-1, respectively. Macro-porous resin enriched product of AE was harmful to mice, with gender differences. Micronucleus rates of each dose(8,16,32 g·kg-1) group and control group for female mouse were 0.28%, 0.34%, 0.26% and 0.22%, respectively. Micronucleus rates of each dose(8,16,32 g·kg-1) group and control group for male mouse were 0.32%, 0.30%, 0.36% and 0.28%, respectively. Sperm shape abnormality rates of each dose group and control group were 3.16%, 3.01%, 2.67% and 3.23%, respectively. Micronucleus rate and sperm shape abnormality rate had no significant increase compared with the negative control. The 30-day repeated intragastric WE and AE had no effect on the general conditions of the model rats. Compared with normal group, AE group showed a significant decrease in heart weight, and significant increases in liver weight, liver index and kidney index (P<0.05). Both of AE and WE group showed significant increases in globulin (GLB) and creatinine (CRE) levels (P<0.05). The transaminases of individual rats were abnormal. Four liver pathological changes were found in necropsy, histopathological examination revealed that liver cells degeneration, necrosis and liver tissue inflammation occurred in the individual rats with abnormalities in biochemical index and organ index.Conclusion: The results indicated that both of WE and AE have potential toxicity. WE does not show any genetic toxicity to mice. Therefore, further studies shall be made for toxicological safety of over-ground parts of G. uralensis.
