Effect of Yangzheng Sanjie Decoction on Proliferation, Apoptosis and ERK Pathway of Human Gastric Cancer MKN-45 Cells
10.13422/j.cnki.syfjx.20190327
- VernacularTitle: 养正散结汤对人胃癌MKN-45细胞增殖、凋亡及ERK通路的影响
- Author:
Zhen-zhen XIAO
1
;
Hai-xia DENG
1
;
Geng-xin CHEN
1
;
Chao-fen QIU
1
;
Ao-qiang ZHOU
1
Author Information
1. The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, China
- Publication Type:Research Article
- Keywords:
Yangzheng Sanjie decoction;
MKN-45 cells;
proliferation;
apoptosis;
extracellular signal-regulated kinase pathway (ERK)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(3):39-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of Yangzheng Sanjie decoction on proliferation, apoptosis and extracellular signal-regulated kinase (ERK) pathway of human gastric cancer MKN-45 cells.Method: Gastric cancer cell line MKN-45 was treated for 24, 48, 72 h with Yangzheng Sanjie decoction (0.5, 1, 1.5, 2, 2.5, 3, 3.5 g·L-1); cell proliferation was measured by cell counting kit-8 (CCK-8); cell colony forming ability was observed by the plate cloning experiment after intervention with Yangzheng Sanjie decoction (0.4, 0.8 g·L-1); MKN-45 cells was treated with 4, 8 g·L-1, and then cell apoptosis was detected by flow cytometry; the expression of ERK and its phosphorylation level were detected by Western blot assay after treatment with 2, 4, 8 g·L-1.Result: Compared with the blank group, Yangzheng Sanjie decoction could significantly inhibit the proliferation of MKN-45 cells. After treatment for 24, 48 h, Yangzheng Sanjie decoction started from 2 g·L-1, and after treatment for 72 h, it started from 1.5 g·L-1, the cell viability gradually decreased in a concentration-dependent manner (P<0.05, P<0.01). After 48 hours of intervention with different concentrations of Yangzheng Sanjie decoction, the cell clone formation rate of Yangzheng Sanjie decoction was significantly lower than that of the blank group with a dose-response relationship (P<0.01). After the intervention with Yangzheng Sanjie decoction at a concentration of 0.8 g·L-1, cell colonies could not be formed; the apoptosis rate of Yangzheng Sanjie decoction was significantly higher than that of the blank group (P<0.05, P<0.01); after 12 h of intervention, compared with the blank group, Yangzheng Sanjie decoction started from 4 g·L-1, and the phosphorylation level of ERK protein in MKN-45 cells was down-regulated (P<0.01).Conclusion: Yangzheng Sanjie decoction can inhibit the proliferation of human gastric cancer cell line MKN-45 and promote its apoptosis. The mechanism may be related to the inhibition of phosphorylation of ERK.
