Therapeutic Effect of Nerve Growth Factor Thermosensitive Gel on Sciatic Nerve Injury of Rats
10.13422/j.cnki.syfjx.20190207
- VernacularTitle: 神经生长因子温敏凝胶对大鼠坐骨神经损伤的修复作用
- Author:
Min JIANG
1
;
Wang-di SONG
1
;
Wen CHEN
1
;
Ya-ke GUO
2
Author Information
1. School of Pharmacy, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, Shihezi University, Shihezi 832000, China
2. The First Affiliated Hospital of the Medical College, Shihezi University, Shihezi 832008, China
- Publication Type:Research Article
- Keywords:
nerve growth factor;
thermosensitive in situ gel;
central composite design-response surface methodology;
sciatic nerve injury;
restorative effect;
sciatic nerve function index;
time of withdrawal reflex
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(3):27-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare nerve growth factor(NGF) temperature sensitive in situ gel and investigate its therapeutic effect on sciatic nerve injury of rats.Method: NGF thermosensitive gel was prepared and its prescription was optimized by central composite design-response surface methodology.Fifty rats were randomly divided into the normal group,model group,NGF injection group(10 mg·L-1),NGF low-dose(10 mg·L-1) and high-dose(20 mg·L-1) thermosensitive gel groups,and sciatic nerve injury model of rats was established.The effect of NGF thermosensitive gel on the injury of sciatic nerve were comprehensively examined by taking rat behavior,sciatic nerve function index(SFI),time of withdrawal reflex,wet weight ratio of gastrocnemius muscle,and histomorphological changes as indicators.Result: The gelation temperature of NGF thermosensitive gel was 35.2℃ after the formulation being optimized,which was in line with the standard for injection.Four-eight weeks after operation,the SFI and wet weight ratio of gastrocnemius muscle in rats of NGF high-dose thermosensitive gel group were significantly higher than those in the model group and NGF injection group,but its time of withdrawal reflex was significantly lower than those in the model group and NGF injection group,and the effect was in a dose-dependent manner.Arrangement of regenerated nerve fibers in sciatic nerve injury area of rats from NGF high-dose thermosensitive gel group was more tidy,dense and continuous than that of the model group.Conclusion: NGF thermosensitive gel can promote repair of sciatic nerve injury in rats.
