Overall Evaluation on Mutual Detoxication Mechanism of Tripterygii Radix et Rhizoma and Lysimachiae Herba by Correlation Analysis of “Chemical Composition Spectrum-attenuation Spectrum-biological Information Spectrum”
10.13422/j.cnki.syfjx.20182206
- VernacularTitle: 从“化学成分谱-减毒作用谱-生物信息谱”关联分析整体评价雷公藤配伍金钱草的相杀减毒作用机制
- Author:
Jun-ming WANG
1
;
Jin-hua LI
1
;
Hong CAI
1
;
Jin-yang LI
1
;
Yue-yue ZHANG
1
;
Jun LI
1
;
Ying CUI
1
Author Information
1. Research and Experiment Center, College of Pharmacy, Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Chinese Medicine, Zhengzhou 450046, China
- Publication Type:Research Article
- Keywords:
mutual detoxication mechanism;
principal component analysis;
gray correlation analysis;
overall evaluation;
"there is no reason why there is no meteorology";
Lysimachiae Herba;
Tripterygii Radix et Rhizoma
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(3):15-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To overall evaluate the mutual detoxication mechanism of Tripterygii Radix et Rhizoma(LGT) compatible with Lysimachiae Herba(JQC) in tumor-bearing state.Method: Twelve differentially characteristic components before and after compatibility were used as chemical composition spectrum,six indicators including serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),creatinine(Cr) and urea nitrogen(BUN),malondialdehyde(MDA) levels in the liver and kidney tissues were used as attenuation spectrum,and twelve biological indicators including glutathione(GSH),glutathione-S-transferase(GST),glutathione peroxidase(GPx),superoxide dismutase(SOD),catalase(CAT) and interleukin(IL)-10 in the liver and kidney were used as the biological information spectrum.Mutual detoxication mechanisms of LGT compatible with JQC in tumor-bearing state were overall evaluated by principal component analysis(PCA),and the contribution of chemical components and biological indicators to mutual detoxication was further evaluated by gray correlation analysis(GCA) of "chemical composition spectrum-attenuation spectrum-biological information spectrum".Result: Compared with the model group,the attenuation spectrum scores Z values of S180(Z1 value) and H22(Z3 value) increased significantly after LGT being used alone(P<0.01).Compared with LGT alone group,the compatibility groups of LGT and JQC significantly reduced excessive Z1 value and Z3 value caused by LGT when the ratio of LGT and JQC was 4:1,2:1,1:1,1:2 and 1:4(P<0.01).The overall efficacy of Z values(Z1 value and Z3 value) of LGT-JQC in the mass ratios including 4:1,1:1,1:2 and 1:4 was significantly higher than that in the ratio of 2:1(P<0.01).LGT also caused a significant decrease in the Z values of the bioinformatics scores in the S180(Z2 value) and H22(Z4 value) tumor-bearing state,these two values were significantly increased after compatibility with JQC.The chemical components contributing the most to the attenuating effect of S180 and H22 in tumor-bearing state were 3# and 10#,respectively.The most important biological indicators were kidney GPx and renal GSH.Conclusion: LGT combined with JQC in the mass ratio of 4:1-1:4 can attenuate LGT-induced subacute toxicity in S180 and H22 tumor-bearing state,and the best ratio of such effect is 2:1.The attenuating effect reflects the thought of "there is no reason why there is no meteorology".The mechanism of attenuating action involves antioxidative damage and anti-inflammatory reaction of the liver and kidney,especially the renal GPx(S180) and renal GSH(H22) as the greatest contribution to the detoxication mechanism.
