Effect of Different Combinations of Gentianae Macrophyllae Radix on MMP-3 and TIMP-1 in Rheumatoid Arthritis Rats with Wind-cold-dampness Arthralgia
10.13422/j.cnki.syfjx.20190334
- VernacularTitle: 秦艽不同配伍对风寒湿痹模型大鼠MMP-3及TIMP-1的影响
- Author:
Shan SU
1
;
Rong WANG
1
;
Min ZHAO
1
;
Chen WU
1
;
Hui-qin GAO
1
Author Information
1. Gansu University of Chinese Medicine, Lanzhou 730000, China
- Publication Type:Research Article
- Keywords:
Gentianae Macrophyllae Radix;
wind-cold-dampness arthralgia;
rheumatoid arthritis (RF);
rheumatoid factor;
matrix metalloproteinase-3(MMP-3);
tissue inhibitor of matrix metalloproteinase-1(TIMP-1)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(3):8-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effects of different combinations of Gentianae Macrophyllae Radix (Qinjiao) on the ankle joint matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) of rheumatoid arthritis (RA) model rats with wind-cold-dampness arthralgia.Method: Eighty healthy SD rats were randomly divided into 8 groups, namely blank control group, collage Ⅱ model group, wind-cold-dampness syndrome model group, positive control group, single-taste Gentianae Macrophyllae Radix group, Gentianae Macrophyllae Radix-Clematidis Radix et Rhizoma group (GC group), Gentianae Macrophyllae Radix-Taxilli Herba group (GT group), Gentianae Macrophyllae Radix-Stephanlae Tetrandrae Radix group (GS group), with 10 rats in each group. Rat model of wind-cold-dampness RA was induced through the injection with type Ⅱ collagen emulsion and wind-cold-dampness stimulation. After the establishment of the model, the blank control group, collage Ⅱ model group and wind-cold-dampness syndrome model group were given normal saline, and the corresponding liquid medicine was given to each administration group. In the experiment, the thickness of the left posterior metatarsal of rats was measured every 3 days, and the swelling degree of metatarsal was calculated. The arthritis index (AI) was evaluated on the 38th day of the experiment. The serum rheamatoid factor(RF) content of rats was detected by enzyme linked immunosorbent assay (ELISA). The expressions of MMP-3 and TIMP-1 in ankle joint were detected by Western blot. The expressions of MMP-3 and TIMP-1 mRNA in ankle joint were detected by real-time fluorescence quantitative PCR (Real-time PCR).Result: Compared with the blank group, the swelling degree, AI score, serum RF content, MMP-3 protein expression and MMP-3 mRNA expression in ankle joints of coll age Ⅱ model group and model wind-cold-dampness syndrome group were significantly increased (P<0.05,P<0.01), while the TIMP-1 protein expression and TIMP-1 mRNA expression in ankle joints were significantly decreased (P<0.01). Compared with the wind-cold-dampness syndrome model group, the swelling degree of foot and metatarsal, AI score and serum RF content of each administration group were decreased to different degrees, especially in Qinwei group (P<0.01). The results of Western blot and Real-time PCR showed that the expressions of MMP-3 protein and MMP-3 mRNA in ankle joints of each administration group were significantly lower than those of wind-cold-dampness syndrome model group (P<0.01). The expressions of TIMP-1 protein and TIMP-1 mRNA in ankle joints of rats in positive control group, GC group and GT group were significantly higher than those in GC group (P<0.01).Conclusion: For rheumatoid arthritis with wind-cold-dampness arthralgia, mild and warm traditional Chinese medicine (TCM) has a better effect than the combination of mild and cold TCM or mild TCM drugs. The experimental results are basically consistent with the principle of "treating cold diseases with hot medicine". The mechanism of the compatibility in treating rheumatoid arthritis due to wind-cold-dampness arthralgia may be related to the reduction of MMP-3, the increase of TIMP-1 expression and the reduction of articular cartilage damage.
