Function of miR-100 on hepatocellular carcinoma invasion and metastasis
10.3760/cma.j.issn.1007-8118.2019.10.002
- VernacularTitle: 微小RNA-100表达对肝癌细胞侵袭和迁移的影响
- Author:
Jianping CAI
1
;
Xianghong ZHOU
2
;
Haibo YU
1
;
Liancai WANG
1
;
Chunhui GAO
1
;
Deyu LI
1
Author Information
1. Department of Hepatobiliary-Pancreatic Surgery, Henan Provincial People's Hospital; People's Hospital of Zhengzhou University, Zhengzhou 453000, China
2. Department of Cardiovascular Medicine, Henan Provincial People's Hospital; Fuwai Central China Cardiovascular Hospital, Zhengzhou 453000, China
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
miR-100;
qRT-PCR;
Invasion;
Metastasis
- From:
Chinese Journal of Hepatobiliary Surgery
2019;25(10):725-727
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of microRNA-100 (miR-100) in hepatocellular carcinoma (HCC) tissues and cells, and further explore the function of miR-100 on invasion and metastasis of HCC.
Methods:A total of 50 patients with HCC from December 2016 to October 2018 in Department of Hepatobiliary Pancreatic Surgery, Henan Provincial People's Hospital were selected, including 32 males and 18 females, aged 20~78 years. PCR was used to detect the expression of miR-100 in HCC tissues, adjacent tissues and HCC cells. HCC cells were transfected with miR-100 sequence, inhibition sequence and corresponding negative control sequence to detect the migration and invasion ability of HCC cells after transfection.
Results:The expression of miR-100 was lowest in SMMC-7721 and MHCC-97H. The relative expression level of miR-100 in HCC tissues was significantly lower than that in normal adjacent liver tissues [(0.39±0.03) vs. (0.56±0.06)], and the difference was statistically significant (P<0.05). Compared with the negative control HCC cells, the migration and invasion of HCC cells with overexpression of miR-100 decreased, while the inhibition of miR-100 expression increased the migration and invasion of HCC cells.
Conclusions:The expression of miR-100 is low in human HCC tissues and cells. miR-100 inhibits the invasion and migration of HCC cells and plays an important role in the occurrence and development of HCC.
