Effects of vitamin C on reversing cisplatin resistance in oral squamous cell carcinoma
10.3872/j.issn.1007-385x.2020.01.005
- VernacularTitle:维生素C逆转口腔鳞状细胞癌顺铂耐药的作用
- Author:
ZHOU Jianjun
1
;
ZHAO Yunfu
1
;
WANG Guodong
1
Author Information
1. (Department of Stomatology, Changzheng Hospital of Naval Military Medical University,
- Publication Type:Journal Article
- Keywords:
oral squamous cell carcinoma (OSCC);
CAL27 cell;
CAL27/DDP cell;
vitamin C (VC);
cisplatin (DDP);
drug resistance;
P-gp
- From:
Chinese Journal of Cancer Biotherapy
2020;27(1):31-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of vitamin C (VC) on reversing cisplatin (DDP) resistance in oral squamous cell carcinoma (OSCC) and the mechanism. Methods: Human OSCC CAL27 cells were cultured in vitro and DDP-resistant CAL27 cell line (CAL27/ DDP) was screened by increasing concentration gradient method. Plate clone formation assay, CCK-8, Wound healing assay, Annexin V-FITC/PI staining flow cytometry were used to determine the effects of DDP alone or in combination with VC on colony formation, proliferation, migration and apoptosis of CAL27/DDP cells. Western blotting was used to detect the expression level of P-gp protein in CAL27 cells, CAL27/DDP cells and VC treated CAL27/DDP cells. Results: The inhibition concentration (IC50) of DDP increased significantly in CAL27/DDP cells as compared with that in CAL27 cells (P<0.05), indicating CAL27/DDP was DDP-resistant.After the combination with VC, the IC50 of DDP on CAL27/DDP cells was significantly reduced compared with that of DDP alone (P<0.05). DDP combined with VC significantly inhibited the migration of CAL27/DDP cells (P<0.01), and promoted the apoptosis rate (P<0.01). The expression level of P-gp protein in CAL27/DDP cells was increased compared with that in CAL27 cells (P<0.05), but decreased after VC intervention (P<0.05). Conclusion: VC can reverse DDP-resistance in OSCC cells by inhibiting P-gp protein expression.
- Full text:20200105.pdf
