GSDME influences sensitivity of breast cancer MCF-7 cells to paclitaxel by regulating cell pyroptosis
10.3872/j.issn.1007-385x.2019.02.002
- VernacularTitle:GSDME通过调控细胞焦亡影响乳腺癌MCF-7细胞对紫杉醇的敏感性
- Author:
SHI Ying
1
;
REN Jingjing
2
;
LIANG Chen
2
;
WANG Fang
2
;
LI Wei
2
;
LI Xiaofu
2
Author Information
1. Clinical Laboratory；b. Department of Laboratory Examination, the ThirdAffiliated Hospital of Zhengzhou University
2. Clinical Laboratory, the ThirdAffiliated Hospital of Zhengzhou University
- Publication Type:Journal Article
- Keywords:
breast cancer;
MCF-7 cells;
pyroptosis;
GSDME;
paclitaxel
- From:
Chinese Journal of Cancer Biotherapy
2019;26(2):146-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate whether GSDME affects the sensitivity of breast cancer MCF-7 cells to paclitaxel (PTX) by regulating cell pyroptosis. Methods: GSDME was knocked-down in MCF-7 cells by RNA interference technique. CCK-8 assay, flow cytometry,lactate dehydrogenase (LDH) release method and Wb were respectively used to detect cell proliferation, pyroptotic rate, LDH release, GSDME-N-terminal protein and cleaved-caspase-3 protein levels in PTX-treated MCF-7 cells before and after GSDME knockdown. Results: Compared with the control group, the pyroptotic rate, LDH release, GSDME-N-terminal protein and cleaved-caspase-3 protein levels in the PTX-treatment group significantly increased (all P<0.01). Compared with the si-NC group, the PTX-sensitivity of si-GSDME group decreased, and the pyroptotic rate, LDH release and GSDME-N-terminal protein all significantly decreased (all P< 0.01). Conclusion: Knock-down of GSDME in MCF-7 cells significantly inhibited cell pyroptosis and reduced drug sensitivity of MCF7 cells to PTX.
- Full text:20190202.pdf
