Study of the effect of polysaccharides from Yupingfeng on keratinocytes
10.3969/j.issn.1006-0111.2019.01.012
- VernacularTitle:玉屏风多糖组分对皮肤角质形成细胞的作用研究
- Author:
Jin HUANG
1
;
Jingxue LIU
;
Ying ZENG
Author Information
1. 上海中医药大学附属岳阳中西医结合医院药学部
- Keywords:
Yupingfeng;
polysaccharide;
keratinocytes;
inflammatory factors
- From:
Journal of Pharmaceutical Practice
2019;37(1):51-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the immunomodulatory effect of Yupingfeng polysaccharides on keratinocytes in vitro.Methods The effects of Yupingfeng polysaccharides on the expression of various inflammatory factors in the activated keratinocytes were detected by the Real-time PCR method.The mRNA and protein expression levels of Filaggrin were detected by Real-time PCR and Elisa respectively.The western bolt assay was used to detect the regulation of NF-κB signaling pathway by polysaccharides.Results Yupingfeng polysaccharides inhibited the secretion of inflammatory factors in the activated keratinocytes, promoted the expression of Filaggrin and exhibited significant inhibitory effect on the NF-κB signaling pathway, which plays an important role in the inflammation and immune response.Conclusion Yupingfeng polysaccharides had an immune regulation effect on skin keratinocytes in vitro and alleviated the development of local inflammation.These results lay a theoretical foundation for the clinical application of Yupingfeng prescription.
