Determination of mexiletine hydrochloride by rose Bengal probe using dual-wavelength resonance light scattering technology
10.16438/j.0513-4870.2019-0538
- VernacularTitle:虎红探针测定盐酸美西律—基于双波长共振光散射技术
- Author:
Hong JIANG
1
;
Xiang-dong PANG
1
;
Ying HU
1
Author Information
1. College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Green Development in Wuling Mountain Areas, Yangtze Normal University, Chongqing 408100, China
- Publication Type:Research Article
- Keywords:
mexiletine hydrochloride;
cetylpyridinium bromide;
rose Bengal;
ual-wavelength;
resonance light scattering
- From:
Acta Pharmaceutica Sinica
2020;55(2):298-304
- CountryChina
- Language:Chinese
-
Abstract:
The resonance light scattering (RLS) spectral characteristics of the interaction between rose Bengal and mexiletine hydrochloride in the presence of cetylpyridinium bromide were investigated. A dual-wavelength resonance light scattering (DWO-RLS) method for the determination of mexiletine hydrochloride in drugs was established. In a weakly acidic solution, rose Bengal interacts with mexiletine hydrochloride and cetylpyridinium bromide to form a red ternary ion association complex, which led to a significantly enhanced resonance light scattering signal and produced two strong characteristic scattering peaks at 372 nm and 596 nm. In these two wavelengths the mass concentration of mexiletine hydrochloride was in the range of 0.004 to 0.65 mg·L-1 and had a good linear relationship with the resonance light scattering enhancement intensity (ΔIRLS), with detection limits of 0.003 2 mg·L-1 (372 nm) and 0.003 8 mg·L-1 (596 nm), respectively. When measured by the dual-wavelength resonance light scattering (DWO-RLS) technique, the detection limit was lower, only 0.001 8 mg·L-1. When the DWO-RLS method was applied to the determination of mexiletine hydrochloride in commercially available mexiletine hydrochloride tablets, and the recovery was 98.5%-103%, and the relative standard deviation was 2.0%-2.7%.
