Echinostoma macrorchis Metacercariae in Cipangopaludina chinensis malleata from Xiengkhuang Province, Lao PDR and Morphologies of Adults from Experimental Animals
10.3347/kjp.2019.57.6.657
- Author:
Woon Mok SOHN
1
;
Byoung Kuk NA
;
Dongmin LEE
;
Keeseon S EOM
;
Tai Soon YONG
;
Jong Yil CHAI
;
Duk Young MIN
Author Information
1. Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 52727, Korea. wmsohn@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
Echinostoma macrorchis;
Cipangopaludina chinensis malleata;
metacercaria;
adult;
Xiengkhuang Province;
Lao PDR;
Chinese mystery snails
- MeSH:
Adult;
Animals;
Asian Continental Ancestry Group;
Crowns;
Digestion;
Echinostoma;
Eggs;
Esophagus;
Female;
Head;
Horns;
Humans;
Metacercariae;
Methods;
Mice;
Ovary;
Ovum;
Pharynx;
Rats;
Seminal Vesicles;
Snails;
Spine;
Testis;
Trematoda
- From:The Korean Journal of Parasitology
2019;57(6):657-664
- CountryRepublic of Korea
- Language:English
-
Abstract:
We identified the echinostome metacercariae in Chinese mystery snails, Cipangopaludina chinensis malleata, from Xiengkhuang Province, Lao PDR with morphologies of adult worms recovered. Total 20 snails were examined with artificial digestion method and then the collected metacercariae were orally infected to a mouse and a rat. Adult worms recovered from experimental animals were observed with a light microscope and a SEM. The metacercariae were round, 125×123 μm in average size, with a moderately thick cyst wall, collar spines distributed in the head collar and excretory granules in 2 canals of excretory tube. Adult flukes (3-week-old in a rat) were elongated, ventrally curved and 5.310×1.023 mm in average size. Head collar distinct, bearing 43 collar spines with 5 end group ones on each side. Oral sucker subterminal, prepharynx very short, pharynx well developed, and esophagus relatively short. Cirrus sac well developed, with a saccular seminal vesicle, and ventral sucker very large. Ovary round and on the median line of the body. Testes tandom and elongated. Eggs operculated, elliptical and 90×57 μm in average size. In the SEM observation, the head crown prominent, with 43 collar spines resembled with horns of younger stag. Scale-like tegumental spines were densely distributed on the surface between the head collar and ventral sucker, and their densities were decreased posteriorly. Conclusively, the metacercariae detected in C. chinensis malleata from Lao PDR were identified as those of Echinostoma macrorchis based on the morphological characteristics of adult worms.
