Expression Level and Clinical Significance of OTC4 Gene in Patients with Myelodysplastic Syndromes.
10.7534/j.issn.1009-2137.2020.01.035
- Author:
Yong-Xiao ZHANG
1
;
Yuan-Yuan CHEN
1
;
Zhi-Shang LI
1
;
Ying-Hua LI
2
,
3
Author Information
1. Department of Hematology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province, China.
2. Department of Hematology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province, China,E-mail: gaozongcai01@
3. com.
- Publication Type:Journal Article
- From:
Journal of Experimental Hematology
2020;28(1):209-213
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression characteristics and clinical value of OTC4 gene in patients with myelodysplastic syndrome (MDS).
METHODS:Sixty-five patients with MDS were selected from June 2017 to April 2018, and 39 healthy subjects were selected as control group. Mononuclear cells were isolated from bone marrow collected by aseptic puncture. The OTC4 gene level of MDS patients was detected by RT-PCR, and the OTC4 protein of MDS patients was detected by Western blot. The survival curve of MDS patients was drawn by Kaplan-Meier. Cox multivariate analysis was used to analyze the independent prognostic factors.
RESULTS:The relative expression level of OTC4 gene in MDS patients was significantly higher than that in the control group (P＜0.05). Western blot showed that the expression level of OTC4 protein in MDS patients was higher than that in the control group (P＜0.05). OTC4 gene expression level closely related with the leukocyte count, and the level of hemoglobin, and lactate dehydrogenase and platelet count in MDS patients (P＜0.05). CR rate of MDS patients with low OTC4 gene expression was 54.8%, which was higher than that of high OTC4 gene expression group (P＜0.05), while HI, SD and PD rates of MDS patients with low OTC4 gene expression were 9.7%, 12.9% and 6.5% respectively, which were lower than those of high OTC4 gene expression group (P＜0.05). Kaplan-Meier survival analysis showed that OS and DFS in patients with low OTC4 gene expression were superior to those with high OTC4 gene expression (P＜0.05). Multivariate Cox regression analysis showed that leukocyte count and OTC4 gene were independent influencing factors for OS (P＜0.05), platelet level and OTC4 gene expression were independent influencing factors for DFS (P＜0.05).
CONCLUSION:OTC4 gene closely relates with the severity of MDS. The patients with lower expression of OTC4 gene have better prognosis, the detection of OTC4 gene has higher clinical value for evaluating the prognosis of MDS patients.
