Effect of the focal adhesion kinase inhibitor TAE226 on the epithelial-mesenchymal transition in human oral squamous cell carcinoma cell line.
- Author:
Xiang-Yu ZOU
1
;
Qin ZENG
1
;
Ping LIU
2
;
Min-Hai NIE
1
Author Information
- Publication Type:Journal Article
- Keywords: epithelial-mesenchymal transition; focal adhesion kinase inhibitor TAE226; oral squamous cell carcinoma
- MeSH: Cadherins; Carcinoma, Squamous Cell; Cell Line, Tumor; Epithelial-Mesenchymal Transition; Focal Adhesion Protein-Tyrosine Kinases; Humans; Morpholines; Mouth Neoplasms; Vimentin
- From: West China Journal of Stomatology 2020;38(1):17-22
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect of the focal adhesion kinase inhibitor TAE226 on epithelial-mesenchymal transition (EMT) in human oral squamous cell carcinoma (OSCC) cell line.
METHODS:HSC-3 and HSC-4 cells were cultured with TAE226 under different concentrations (0, 1, 5, and 10 μmol·L⁻¹) for 24, 48, and 72 h. Real-time quantitative polymerase chain reaction was performed to detect the mRNA expressions of E-cadherin and Vimentin. The protein expressions of E-cadherin and Vimentin were determined by Western blot assay after 48 h of TAE226 treatment.
RESULTS:Real-time quantitative polymerase chain reaction showed that increasing the TAE226 dose and reaction time resulted in increased and decreased E-cadherin and Vimentin mRNA expressions, respectively (P<0.05). Western blot assays showed that increasing the TAE226 dose resulted in increased and decreased E-cadherin and Vimentin protein expressions, respectively (P<0.05).
CONCLUSIONS:TAE226, which is expected to be an effective drug for OSCC treatment, can effectively inhibit the EMT of the OSCC cell line.
