Preparation and in vitro evaluation of phospholipid-coated silver-graphene quantum dot multifunctional nanoparticles
10.16438/j.0513-4870.2018-1054
- VernacularTitle:磷脂包覆银-石墨烯量子点多功能纳米粒的制备与体外评价
- Author:
Qiao-bei PAN
1
;
Jing ZHANG
2
;
Xiang LI
1
;
Yuan-ying FANG
3
;
Yi JIN
3
Author Information
1. State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine, Nanchang 330028, China
2. Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330028, China
3. National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
- Publication Type:Journal Article
- Keywords:
silver nanoparticles;
phospholipid;
cytotoxicity;
graphene quantum dot;
fluorescence imaging
- From:
Acta Pharmaceutica Sinica
2019;54(2):366-372
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, multifunctional silver-graphene quantum dot nanoparticles coated with phospholipids (ADG-DDPC) were prepared and their properties were evaluated in vitro. Cationic phospholipids 1,2-diolefinoxy-3-trimethy-laminopropane (DOTAP) was absorbed first onto the surface of the core of silver nanoparticle (AgNPs) through the mutual attraction between the positive and negative charge. Based on the principle of phase transformation and hydrophobic interaction, dstearyl-phosphatidylglycolamine-polyethylene-glycol-cyclic-cRGD peptide (DSPE-PEG2000-cRGD) self-assembled onto the outlayer of DOTAP of AgNPs. A stable multifunctional nano-preparation was formed and its ultraviolet absorption, particle size distribution, morphology,in vitro release behavior, ability to kill cancer cells and cell uptake were studied. The maximum UV absorption of the synthesized nanometer preparation was about 400 nm. Malvern particle size meter and transmission electron microscope showed that the particle size of the nano- preparation was about 30-40 nm and its particle size distribution was uniform. The in vitro release of nano-preparation was positively correlated with the concentration of H2O2. The IC50 value of AgNPs for tumor cells was (347.78 ± 0.06) ng·mL-1, and the IC50 value of ADG-DDPC for tumor cells was (209.68 ± 0.09) ng·mL-1, indicating that ADG-DDPC possessed a stronger cytotoxicity than that of AgNPs. Cell uptake experiment showed that ADG-DDPC could be absorbed by tumor cells and exhibited fluoresce inside those cells. In conclusion, ADG-DDPC was successfully prepared, and in vitrocharacterization study pointed to that the nano-preparation exhibits a higher antitumor activity than AgNPs.
