Quality control of Shuanghuanglian preparations using an effect-constituent index
10.16438/j.0513-4870.2019-0449
- VernacularTitle:基于效应成分指数的双黄连制剂质量控制研究
- Author:
Hui ZHANG
1
;
Si-yu LI
1
;
Hong-ling FENG
1
;
Mu-rui LI
1
;
Zhi-dong PEI
1
;
Ting-guo KANG
1
Author Information
1. College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
- Publication Type:Research Article
- Keywords:
Shuanghuanglian preparation;
effect-constituent index;
antibacterial potency;
quantitative fingerprint chromatogram;
quality control
- From:
Acta Pharmaceutica Sinica
2019;54(12):2149-2154
- CountryChina
- Language:Chinese
-
Abstract:
We established a quality evaluation method for Shuanghuanglian preparations based on an effect-constituent index (ECI), which is guided by the clinical efficacy of Shuanghuanglian and a dose-efficacy correlation. An HPLC method was used to establish the quantitative fingerprint of Shuanghuanglian from different manufacturers and to determine the content of 10 fingerprint components, including baicalin, chlorogenic acid, forsythin, galuteolin, wogonin, forsythoside A, luteolin, caffeic acid, baicalein, and scutellarin. Using Staphylococcus aureus as biological model, the potency of Shuanghuanglian preparations was determined by antibiotic microbial assay. Using the method of PLC-DA, the efficacious antibacterial components were screened by "dose-efficacy" correlation analysis. According to the antibacterial potency and content of the antibacterial ingredients, combined with the method of the custom weight coefficient, ECI was calculated and verified. The results show that the antibacterial ECI can facilitate evaluation of the efficacy of Shuanghuanglian based on the composition of its contents, providing a new method for the quality control of traditional Chinese medicine.
