Differential pathway and gene expressions of endophytic Phialocephala fortinii inoculated on Rhodiola crenulata
10.16438/j.0513-4870.2018-0709
- VernacularTitle:内生菌Phialocephala fortinii影响大花红景天代谢的差异途径及基因表达
- Author:
Meng-liang WANG
1
;
Xiao-yin GAO
1
;
Jin-long CUI
1
;
Jun-hong WANG
1
Author Information
1. Institute of Applied Chemistry, Taiyuan 030006, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Phialocephala fortinii;
RNA-Seq;
internal reference genes;
real-time fluorescent quantitative PCR;
plant-fungus interaction
- From:
Acta Pharmaceutica Sinica
2018;53(12):2122-2128
- CountryChina
- Language:Chinese
-
Abstract:
The development and metabolism of medicinal plant is affected by many factors, among which the effect from endophytic fungi has been noticed recently and has become one of hot fields. In order to explore the effect of endophytic fungi on gene expression in R. crenulata, RNA-sequencing was used to find genes involved in metabolic pathways, and the differential genes were verified by real-time fluorescent quantitative PCR. The method of 2-△△Ct was used to analyze the relative expression levels of genes in related metabolic pathways, which was used to verify the result of transcriptomics sequencing. The results showed that the endophytic fungus, P. fortinii, could up-regulate the gene expression in lipid metabolic pathway of R. crenulata. In signal transduction pathway, the genes were influenced at different level but the gene expressions were significantly increased under control of Notch signaling pathway, which was 34 times of that in control. The gene expressions of environmental adaption pathway were up-regulated in R. crenulata after inoculation of P. fortinii. This study could provide help for further understanding on mechanism of plant-fungus interaction, root cause of geoherbalism of medicinal plant and exploring bio-function of endophytic fungi.
