Modification of 6 Hz corneal kindled mouse model for drug-resistant epilepsy and its treatment by three traditional Chinese medicine prescriptions
10.16438/j.0513-4870.2018-0158
- VernacularTitle:6 Hz角膜点燃耐药癫痫小鼠模型改良及3种中药方剂的作用
- Author:
Hai-mei LU
1
;
Mei-juan XIE
1
;
Shan LI
1
;
Jie YU
1
;
Jia ZHOU
1
;
Zhong CHEN
1
;
Zheng-hao XU
1
Author Information
1. Basic Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China
- Publication Type:SPECIAL REPORTS
- Keywords:
epilepsy;
drug resistance;
drug resistant epilepsy;
kindling;
traditional Chinese medicine
- From:
Acta Pharmaceutica Sinica
2018;53(7):1048-1053
- CountryChina
- Language:Chinese
-
Abstract:
Drug-resistance is a challenge in the treatment of epilepsy, and traditional Chinese medicine (TCM) prescription may have a potential in therapy of the epilepsy. Here we established a modified 6 Hz corneal kindled mouse model of epilepsy, and verified its drug-resistance to four commonly used western medicines. We evaluated the efficacy of three classical TCM prescriptions in this drug-resistant epilepsy model. The results showed that:① most C57BL/6J mice with stimulation current intensity at 44 mA (8 out of 10) were fully kindled, while none ICR mice with stimulation current intensity at 44 mA or C57BL/6J mice with stimulation current intensity at 24 mA were fully kindled. Fully kindled mice exhibited epileptic electroencephalograms after 44 mA and 6 Hz corneal kindling stimulation and increased activation of astrocytes in the hippocampus (by staining the glial fibrillary acidic protein); ② 50 mg·kg-1 phenytoin sodium, 100 mg·kg-1 valproic acid sodium and 15 mg·kg-1 lamotrigine had no significant effects on the drug-resistant seizures in 6 Hz corneal kindled C57BL/6J mice, while 100 mg·kg-1 levetiracetam significantly reduced the seizure stage (P < 0.05) and incidence of generalized seizure (P < 0.05) in it; ③ TCM prescriptions, Chai-Hu Plus Long-Gu Mu-Li decoction (9.36 or 28.08 g·kg-1) and Tian-Ma Gou-Teng decoction (14.82 or 44.46 g·kg-1), had no significant effects on the drug-resistant seizures in 6 Hz corneal kindled C57BL/6J mice, while TCM prescription Feng-Yin decoction (19.11 g·kg-1) significantly reduced both the seizure stage (P < 0.01) and incidence of generalized seizure (P < 0.05) in it. Thus, the modified 6 Hz corneal kindled C57BL/6J mouse model is an excellent drug-resistant epilepsy model, Feng-Yin decoction have antiepileptic effects on it, suggesting Feng-Yin decoction may be an effective TCM prescription for clinical treatment of drug-resistant epilepsy.
