Co-culture of human breast adenocarcinoma cells and human umbilical vein endothelial cells to mimic in vivo tumor microenvironment
10.16438/j.0513-4870.2017-1205
- VernacularTitle:模拟体内肿瘤微环境的乳腺癌细胞与脐静脉内皮细胞的体外共培养
- Author:
Yan-fang YANG
1
;
Ying-ying MENG
1
;
Jun YE
1
;
Xue-jun XIA
1
;
Lin LI
1
;
Wu-jun DONG
1
;
Hong-liang WANG
1
;
Yu-ling LIU
1
Author Information
1. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
tumor microenvironment;
human breast adenocarcinoma cell;
human umbilical vein endothelial cell;
double-layered cells model;
non-contact co-culture
- From:
Acta Pharmaceutica Sinica
2018;53(3):403-409
- CountryChina
- Language:Chinese
-
Abstract:
The development of tumor tissue is a complicated process, which is closely related to tumor microenvironment. In order to simulate the tumor tissue in vivo, non-contact co-culture of human breast adenocarcinoma cells (MCF-7 cells) and human umbilical vein endothelial cells (HUVECs cells) using transwell cell culture plate was developed in this study. The cell viability, morphology, cell resistance, cell cycle and vascular endothelial growth factor (VEGF) protein content of co-cultured MCF-7 and HUVECs cells were investigated, and compared with those of separately cultivated MCF-7 and HUVECs cells during the same period. Different to the separately cultured MCF-7 and HUVECs cells, co-cultured MCF-7 and HUVECs cells exhibited higher cell viability, deformed cell morphology, lower cell resistance, higher proportion of S and G2/M phases and higher VEGF protein content (about 1.4−2 times). The double cell model via non-contact co-culture of MCF-7 and HUVECs cells constructed in this study could simulate the interaction between tumor cells and tumor vascular endothelial cells in vivo, which may provide a more realistic model for subsequent study of drug release system in the control of breast cancer in vitro.
