Preparation and evaluation of photo-sensitive reactive oxygen species responsive liposomes
10.16438/j.0513-4870.2017-0864
- VernacularTitle:光敏活性氧自由基响应脂质体的制备和评价
- Author:
Guang-zhao LU
1
;
Cheng HOU
1
;
He ZHANG
1
;
Zhi-guo SUN
1
;
Ying LU
1
;
Yan-qiang ZHONG
1
;
Hao ZOU
1
Author Information
1. School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
photo-sensitive;
reactive oxygen species;
liposome;
photosensitizer;
singlet oxygen
- From:
Acta Pharmaceutica Sinica
2017;52(12):1942-1950
- CountryChina
- Language:Chinese
-
Abstract:
Photo-sensitive reactive oxygen species (ROS) responsive liposomes loaded with 1,4,8,11,15, 18,22,25-octabutoxypalladium phthalocyanine[PdPC(OBu)8] and doxorubicin hydrochloride (DOX) (LPD) were prepared by (NH4)2SO4-gradient method. LPD was characterized with transmission electron microscopy (TEM), dynamic light scattering particle size, zeta potentials, photo-sensitive ROS-responsive DOX release behaviors and the serum stability in vitro. LPD cytotoxicity, DOX uptake and singlet oxygen production in MCF-7 cells were evaluated. The results showed that the particle size of LPD was (169.3 ±1.2) nm, PDI of LPD was 0.198 ±0.003 and zeta potentials of LPD was (-39.8 ±0.8) mV. The accumulated release of DOX reached 95.5% in 5 min under 730 nm laser irradiation (300 mW·cm-2). The DOX uptake of liposome was increased and 1O2 was generated. The half inhibition concentration (IC50) of DOX in LPD with irradiation group was decreased by 85.7% and no irradiation group was decreased by 67.9% compared with free DOX group in MCF-7 cells. Therefore, photo-sensitive ROS-responsive liposomes would be a promising drug delivery system for tumor therapy.
