Preparation and evaluation of anti-tumor metastasis of self-assembly micelles loaded with indocyanine green in vitro
10.16438/j.0513-4870.2017-0848
- VernacularTitle:载吲哚菁绿和多柔比星自组装胶束的构建及体外抗肿瘤及其转移的评价
- Author:
Jia-jing TANG
1
;
Ling MEI
1
;
Qian-wen YU
1
;
Qin HE
1
Author Information
1. Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
micelle;
low molecular weight heparin;
cancer therapy;
drug delivery
- From:
Acta Pharmaceutica Sinica
2017;52(12):1933-1941
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we used low molecular weight heparin (LH) as hydrophilic sides, doxorubicin (DOX) as hydrophobic sides, and indocyanine green (ICG) as a photosensitive drug to prepare pH-sensitive self-assembled polymeric micelles (LH-DOX/ICG). The micelles were prepared by dialysis, and evaluated for stability, pH sensibility in vitro, hemolytic test and photo-thermal effect. Wound healing test was used to evaluate the anti-metastatic effects. MTT assay and apoptosis detection kit were used to evaluate the cytotoxicity of micelles against melanoma B16F10 cells. The size of the micelles was (148.7 ±2.1) nm and the zeta potential was (-30.7 ±1.1) mV. The drug-loading content of DOX and ICG were 11.82% and 5.59%, respectively. The micelles exhibited spherical in shape, fairly uniform size. The micelles were stable within 48 h in 50% fetal bovine serum phosphate buffer. The release of DOX was pH-sensitive, while the release of ICG was sustained. The micelles were biocompatible and safe as indicated by the hemolytic test. In vitro photo-thermal effect indicated LH-DOX/ICG micelles had similar photo-thermal effect to the free ICG. Wound healing test showed that the micelles had a good ability to inhibit tumor migration with laser irradiation. MTT assay and cell apoptosis assay indicated that LH-DOX/ICG micelles displayed more efficient anti-tumor effect after near infrared laser compared to LH-DOX micelles. LH-DOX/ICG micelles are promising for the therapeutic effect of phototherapy and chemotherapy in combination for melanoma.
