Study on droplet microfluidic chips for drug screening against Candida albicans
10.16438/j.0513-4870.2017-0743
- VernacularTitle:基于液滴微流控芯片技术的抗白念珠菌药物筛选研究
- Author:
Zhen ZHENG
1
;
Yang CHEN
1
;
Wu-hong LI
1
;
Zhen-yu ZHU
1
;
Zhan-ying HONG
1
;
Yi-feng CHAI
1
Author Information
1. School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
droplet microfluidic chips;
Alamar blue;
antifungal drug screening;
Candida albicans;
iodiconazole
- From:
Acta Pharmaceutica Sinica
2017;52(12):1884-1889
- CountryChina
- Language:Chinese
-
Abstract:
A droplet microfluidic chip system was developed for drug screening against Candida albicans. The microfluidic chip was designed and prepared for the formation of droplets. Alamar blue was selected as an indicator for its characteristic of fluorescence mission in live cells. Four antifungal drugs (amphotericin B, caspofungin, 5-fluorocytosine, terbinafine) and a new drug (iodiconazole) were selected as model drugs to test the microfluidic chip approach. At the same time, 96-well microplate method was performed to verify the applicability of the chip method. The results showed that the developed droplet microfluidic chip platform was able to complete the antifungal susceptibility test within 2 h. In comparison with the 96-well microplate method, the microfluidic chip method showed a consistence of 100% with regard to the minimum inhibition concentrations and less reagent consumption. The new droplet microfluidic chip method is simple, rapid and suitable for rapid screening of antifungal drugs.
