Rapid identification of chemical constituents in Huangqi Jianzhong Tang by UHPLC coupled with hybrid quadrupole-orbitrap MS
10.16438/j.0513-4870.2016-1191
- VernacularTitle:UHPLC-Q Exactive轨道肼高分辨质谱在线快速识别黄芪建中汤的化学成分
- Author:
Ying-huan HU
1
;
Wen-qian XU
1
;
Xue-mei QIN
1
;
Yue-tao LIU
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Huangqi Jianzhong Tang;
chemical constituent;
UHPLC-Q Exactive quadrupole-orbitrap MS
- From:
Acta Pharmaceutica Sinica
2017;52(6):964-970
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to establish an ultra-liquid chromatography-mass spectrometry method for the reliable identification the multiple chemical components in Huangqi Jianzhong Tang (HQJZ). The ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry method was applied to identify the chemical constituents in the HQJZ rapidly. A total of 71 compounds including two major categories of saponins and flavonoids were identified or tentatively deduced on the basis of their retention behaviors, fragments of multistage mass spectrometry or by comparing with reference substances and literatures. Among them, 20 compounds were from Astragali Radix, 14 were from Paeoniae Radix Alba, 37 were from Glycyrrhizae Radix et Rhizoma Praeparata cum Melle, 3 were from Rhizoma Zingiberis Recens, 2 were from Cinnamomi Ramulus and Jujubae Fructus, respectively. The LC-MS method was used to qualitatively analyze the chemical constituents of HQJZ, which provides a scientific basis for the quality control of HQJZ.
