The inhibitory effects of calpain inhibitors on epithelial-mesenchymal transition of MCF-10A mammary epithelial cells induced by fibronectin
10.16438/j.0513-4870.2016-1181
- VernacularTitle:钙蛋白酶抑制剂对纤连蛋白诱导的MCF-10A乳腺上皮细胞-间质转化的影响
- Author:
Duan-yang HONG
1
;
Lin CHEN
1
;
Yan-yan ZHANG
1
;
Jing WANG
1
;
Chang-quan WANG
1
;
Yan CHEN
1
;
Xiang-chun SHEN
1
Author Information
1. Key Laboratory of Optimal Utilization of Natural Medical Resources, Guizhou Medical University, Guiyang 550025, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
calpain inhibitor;
MCF-10A mammary epithelial cells;
fibronectin;
epithelial-mesenchymal transformation;
calpain
- From:
Acta Pharmaceutica Sinica
2017;52(4):569-574
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this research is to investigate the inhibitory effects of calpain inhibitors (ALLN and calpain inhibitor IV) on mammary epithelial-mesenchymal transition (EMT) of MCF-10A cells induced by fibronectin (FN). After FN treatment of MCF-10A cells for 48 h, cell migration and invasion were determined by scratch repair assay and matrigel coated transwell assay, respectively. Vimentin, E-cadherin, snail and calpain-2 protein expression were measured by Western blot. The results suggest that FN induced morphological changes in MCF-10A cells, significantly increased the migration and invasion abilities of MCF-10A cells, upregulated the expression of calpain-2, vimentin and snail, and downregulated the expression of E-cadherin. All such changes by FN were reversed with ALLN and calpain inhibitor IV. In conclusion, the upregulation of calpain-2 was involved in FN-induced EMT of MCF-10A mammary epithelial cells, which may be inhibited by ALLN and calpain inhibitor IV.
