Rapid identification of the different constituents in Fructus Schisandrae Chinensis before and after processing by UHPLC-QTOF/MSE combining with metabonomics
10.16438/j.0513-4870.2016-0151
- VernacularTitle:UHPLC-QTOF/MSE与代谢组学技术对北五味子炮制前后化学成分迁移研究
- Author:
Wei LI
1
;
Yong-gui SONG
1
;
Kuang-yi LIU
1
;
Lin-jun YANG
1
;
Ya-li LIU
1
;
Dan SU
1
;
Yu-lin FENG
1
Author Information
1. Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
Fructus Schisandrae Chinensis;
wine-processed;
vinegar-processed;
UHPLC-QTOF/MSE;
partial least squares-discriminant analysis;
chemical composition
- From:
Acta Pharmaceutica Sinica
2016;51(9):1445-1450
- CountryChina
- Language:Chinese
-
Abstract:
This study was performed to use UHPLC-QTOF/MSE technology to rapidly search and identify variations of chemical ingredients between Fructus Schisandrae Chinensis and its processed products. The present study provides a basis for the study of Chinese herbal medicine processing with a focus on the impact of processing on chemical components. Using a time-dependent data scan mode (MSE) couple with metabolomics technology, we acquired accurate data and identified the potential chemical markers. A total of 12 chemical markers were identified in the crude, vinegar-processed and wine-processed Schisandra chinensis fruit; The results showed that the levels of 6-O-benzoylgomisin O, schisantherin B, schisantherin C, schisantherin D and neokadsuranic acid are the highest in crude Schisandra chinensis fruit; thelevels of schizandrin A, schizandrin B, schizandrin C, gomisin D and gomisin T are the highest in wine-processed Schisandra chinensis fruit; the levels ofschisantherin A and schisandrin are the highest in vinegar-processed Schisandra chinensis fruit. There were significant changes of chemical components between Fructus Schisandrae Chinensis and their processed products, and these findings may offer a reasonable explanation for variation of efficacy and clinical applications in the processed products of Fructus Schisandrae Chinensis.
