Rapid characterization of constituents absorbed into blood after oral administration of steroidal saponins from Radix Ophiopogonis using UHPLC-LTQ-Orbitrap MS coupled with higher energy collision induced dissociation
10.16438/j.0513-4870.2016-0326
- VernacularTitle:UHPLC-LTQ-Orbitrap MS结合高能碰撞诱导裂解技术快速鉴定大鼠口服麦冬甾体皂苷后的血中移行成分
- Author:
Ying LIU
1
;
Wei CAI
2
;
Ning LI
3
;
Si-yi LIU
1
;
Zi-jian WANG
1
;
Zhen-qing LIU
1
;
Jia-yu ZHANG
1
;
Bao-sheng ZHAO
1
Author Information
1. Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
2. Dong Pharmaceutical Research of Hunan Key Laboratory, Hunan University of Medicine, Huaihua 418000, China
3. HKUST Shenzhen Research Institute, Shenzhen 518057, China
- Publication Type:ORIGINAL ARTICLES
- Keywords:
UHPLC-LTQ-Orbitrap MS;
high energy collision dissociation;
steroidal saponins;
constituents absorbed into blood;
Radix Ophiopogonis
- From:
Acta Pharmaceutica Sinica
2016;51(11):1751-
- CountryChina
- Language:Chinese
-
Abstract:
The method of UHPLC-LTQ-Orbitrap mass spectrometry coupled with higher energy collision dissociation (HCD) was established to rapidly analyze the constituents absorbed into blood after oral administration of steroidal saponins from Radix Ophiopogonis. A total of 31 constituents, including 13 furostanol steroidal saponins and 18 spirostanol steroidal saponins, were characterized based on the accurate mass measurements, fragmentation patterns, chromatographic retention times, and diagnostic product ions. Among them, 8 compounds were unambiguously identified by comparison with their corresponding standards. The results provide comprehensive insights and guidance for elucidation of material basis of Radix Ophiopogonis activity.
