Morphological analysis of autophagy
10.16438/j.0513-4870.2015-0876
- VernacularTitle:形态学检测在自噬研究中的应用
- Author:
Fang HUA
1
;
Zhuo-wei HU
1
Author Information
1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Institute of Meteria Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
- Publication Type:SPECIAL REPORTS
- Keywords:
autophagy;
transmission electron microscopy;
immunofluorescence;
microtubule-associated protein 1A/1B-light chain 3;
WD repeat domain phosphoinositide-interacting protein 1;
Keima
- From:
Acta Pharmaceutica Sinica
2016;51(1):39-
- CountryChina
- Language:Chinese
-
Abstract:
Autophagy is an important homeostatic cellular recycling mechanism responsible for degrading injured or dysfunctional subcellular organelles and proteins in all living cells. The process of autophagy can be divided into three relatively independent steps: the initiation of phagophore, the formation of autophagosome and the maturation/degradation stage. Different morphological characteristics and molecular marker changes can be observed at these stages. Morphological approaches are useful to produce novel knowledge that would not be achieved through other experimental methods. Here we summarize the morphological methods in monitoring autophagy, the principles in data interpretation and the cautions that should be considered in the study of autophagy.
