Delivery of miR-375 and doxorubicin hydrochloride by lipid bilayer coated hollow mesoporous silica nanoparticles for liver cancer therapy
10.16438/j.0513-4870.2018-0747
- VernacularTitle:脂质-中空介孔硅联合递送盐酸多柔比星及miR-375治疗肝癌的研究
- Author:
Zhao-yang YU
1
;
Hui-ying XUE
2
;
Lin QIU
2
;
Yi LIU
2
;
Juan LI
2
Author Information
1. Department of Pharmacy, Tongji Hospital; School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
2. Department of Pharmacy, Tongji Hospital
- Publication Type:Research Article
- Keywords:
oxorubicin;
miR-375;
rug delivery system;
hollow mesoporous silicon;
nanocarrier
- From:
Acta Pharmaceutica Sinica
2019;54(1):151-158
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to prepare a novel lipid bilayer coated hollow mesoporous silica nanocarrier for co-delivery of gene drugs and chemotherapeutic drugs to enhance the inhibitory activity of antitumor drugs in hepatoma cells. Hollow mesoporous silica was synthesized by modified StÖber method. Lipid-fusion principle was used to prepare lipid-hollow co-loaded doxorubicin (DOX) and miR-375 (LHMSN-DOX/miR-375). Meanwhile, the morphology, particle size, surface potential, drug loading and release were characterized in vitro. The inhibition of cell proliferation, cell migration and invasion was then evaluated. The results indicated that the core-shell structure of LHMSN-DOX/miR-375 was clear with an intact outer lipid membrane and an ordered internal HMSN mesoporous structure. The drug release amount was pH responsive while the drug was rapidly released under simulated intracellular acidic conditions relative to normal physiological environment. Compared with free DOX, LHMSN-DOX/miR-375 can deliver DOX and miR-375 to liver cancer cells and inhibit the proliferation, migration and invasion of cells more effectively.
