Characteristics of CD180 Expression and Its Diagnostic Value in B Cell Chronic Lymphoproliferative Disorders.
10.7534/j.issn.1009-2137.2018.06.038
- Author:
Jing WU
1
;
Yan SHENG
1
;
Jing-Ni SUI
1
;
Xiang-Qin WENG
2
,
3
Author Information
1. Shanghai Institute of Hematology，Ruijin Hospital Affiliated Shanghai Jiaotong University School of Medicine，Shanghai，200025，China.
2. Shanghai Institute of Hematology，Ruijin Hospital Affiliated Shanghai Jiaotong University School of Medicine，Shanghai，200025，China.E-mail：wxq7803@
3. com.
- Publication Type:Journal Article
- MeSH:
Antigens, CD;
analysis;
B-Lymphocytes;
Biomarkers;
Flow Cytometry;
Humans;
Immunophenotyping;
Leukemia, Lymphocytic, Chronic, B-Cell;
Lymphoproliferative Disorders
- From:
Journal of Experimental Hematology
2018;26(6):1811-1815
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the charactcristics of CD180 expression and differentiation diagnostic value in B cell chronic lymphoproliferative disorders (B-CLPD) through detecting the mean fluorescence intensity（MFI）of CD180 in different sub types of B-CLPD，using multiparameter flow cytometry (FCM).
METHODS:The CD180 MFI of malignant B cells in 178 patients with B-CLPD was detected by FCM. The level of CD180 MFI in various types of B-CLPD was compared to the normal control group. The level of CD180 MFI among sub-types of B-CLPD was also compared.
RESULTS:(1) The expression levels of CD180 in B-CLPD was significantly lower as compared with the normal controls，except the spleen difuse red pulp lymphoma (SDRPL); (2) The CD180 MFI in chronic lymphocytic leukemia sCLL) was significantly lower as compared with other B-CLPD cells; (3) CD180 ware significantly overexpressed in HCL compared with MCL，LPL，and MZL (P <0.05); (4) In the spleen-derived B-CLPD，such as SMZL，HCL, HCL variation and SDRPL，the expression of CD180 has significant difference between lymphomas with or without villous.
CONCLUSION:Utilizing the multiparameter flow cytometry for defecting expression of CD180 and other immunological markers can more efficiently distinguish the subtypes of B-CLPD.
