Icariin protects vascular endothelial cells from oxidative stress through inhibiting endoplasmic reticulum stress.
10.1016/j.joim.2019.01.011
- Author:
Fang-Yuan WANG
1
;
Jian JIA
1
;
Huan-Huan SONG
1
;
Cheng-Ming JIA
1
;
Chang-Bo CHEN
1
;
Jing MA
2
Author Information
1. Department of Traditional Chinese Medicine, Army Medical Center in Traditional Chinese Medicine, Xijing Hospital, Forth Military Medical University, Xi'an 710032, Shaanxi Province, China.
2. Department of Traditional Chinese Medicine, Army Medical Center in Traditional Chinese Medicine, Xijing Hospital, Forth Military Medical University, Xi'an 710032, Shaanxi Province, China. Electronic address: jingma@fmmu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Endoplasmic reticulum stress;
Human umbilical vein endothelial cells;
Icariin;
Oxidative stress
- From:
Journal of Integrative Medicine
2019;17(3):205-212
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the protective effect and underlying mechanism(s) of icariin (ICA) in preventing hydrogen peroxide (HO)-induced vascular endothelial cell injury via endoplasmic reticulum stress (ERS).
METHODS:To study the effects of ICA on HO-induced damage, we used the cell counting kit-8 assay to detect cell viability and the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay to determine cell adhesion and apoptosis, respectively. Spectrophotometry and enzyme-linked immunosorbent assay were used to measure the expression levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Subsequently, glucose-regulated protein 78 (GRP78), activating transcription factor-4 (ATF4) and eukaryotic initiation factor-2α (eIF2α) were detected using Western blotting.
RESULTS:In human umbilical vein endothelial cells, different concentrations of ICA exhibited multiple effects, including reduced HO damage, improved cell viability and adhesion, reduced cell apoptosis and increased SOD and GSH-Px activity. Among the ICA concentrations used, only the HO + 100 μmol/L ICA group had significant differences compared to the HO group. ERS activators HO and dl-dithiothreitol (DTT) significantly increased GRP78, ATF4 and eIF2α expressions, decreased cell activity and reduced SOD and GSH-Px activity. In contrast, the HO + 100 μmol/L ICA and HO + 100 μmol/L ICA + DTT groups had significant inhibitory effects on the expressions of GRP78, ATF4 and eIF2α proteins, showing enhanced cell viability and SOD and GSH-Px activity.
CONCLUSION:The results showed the dose-dependent effects of ICA against HO-induced injury in vascular endothelial cells. The inhibition of GRP78, ATF4 and eIF2α protein expressions in the ERS, and the subsequent alleviation of oxidative stress damage, might be the molecular mechanism.
