Effects of TGF-β1 on the expression and secretion of cytokines induced by Aβ in hippocampal neuron and microglia co-cultures.
10.12047/j.cjap.5668.2018.088
- Author:
Xiao-Xia FANG
1
;
Yi ZHOU
1
;
Gao-Lin SUN
1
;
Yi-Hua QIU
1
;
Yu-Ping PENG
1
Author Information
1. Department of Physiology, School of Medicine, Nantong University, Nantong 226001, China.
- Publication Type:Journal Article
- Keywords:
Aβ1-42;
TGF-β1;
cytokines;
hippocampal neurons;
microglia
- MeSH:
Animals;
Cells, Cultured;
Coculture Techniques;
Cytokines;
Hippocampus;
Microglia;
Neurons;
Rats;
Rats, Sprague-Dawley;
Transforming Growth Factor beta1;
Tumor Necrosis Factor-alpha
- From:
Chinese Journal of Applied Physiology
2018;34(5):385-388 395
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the neuroprotective effects of transforming growth factor beta 1(TGF-β1) on the expression and secretion of cytokines induced by Aβ in hippocampal neurons and microglial co-cultures.
METHODS:Hippocampal neurons and microglia obtained from SD rat were co-cultured. TGF-β1 was applied on day 5 after the neurons and microglia co-cultures were incubated at the concentrations of 5 or 20 ng/ml, Aβ was added 1 h following TGF-β1 application at a concentration of 5 μmol/L. They were incubated for 72 h and then assessed for further studies. Western blot analyses were employed to examine the expression of inducible nitric oxide synthase (iNOS); Real-time PCR and ELISA were used to detect the mRNA expression and secretion of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and insulin-like growth factor-1 (IGF-1).
RESULTS:In the hippocampal neuron-microglia co-cultures, Aβ induced upregulation of iNOS, TNF-α and IL-1β, downregulation of IGF-1. TGF-β1 pretreatment ameliorated the pro-inflammatory effects caused by Aβ.
CONCLUSIONS:TGF-β1 significantly inhibits the increase in inflammatory cytokines and the decrease in neurotrophic factor which are caused by Aβ-induced microglia activation.
