Placenta-derived mesenchymal stem cells with HLA-G positive expression induce Treg .
10.12047/j.cjap.5621.2018.090
- Author:
Gui-Yu CUI
1
;
Jian BAI
2
;
Lan-Ying MIAO
2
;
Da-Yong LIN
2
;
Hong LIU
2
;
Ya-Li LI
3
;
Xi-Cheng LIU
3
Author Information
1. Medical School of Inner Mongolia University for Nationalities, Tongliao
2. Liao Ning University of Traditional Chinese Medicine, Shenyang
3. Department of Anesthesiology, Shenzhen People's Hospital, Shenzhen
- Publication Type:Journal Article
- Keywords:
T lymphocyte;
human leucocyte antigen-G;
placenta-derived mesenchymal stem cell;
regulatory T lymphocyte
- MeSH:
Female;
Forkhead Transcription Factors;
HLA-G Antigens;
Humans;
Mesenchymal Stem Cells;
Placenta;
Pregnancy;
T-Lymphocytes, Regulatory
- From:
Chinese Journal of Applied Physiology
2018;34(5):396-400
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study placenta-derived mesenchymal stem cells with HLA-G (Human Leukocyte Antigen, HLA-G) positive expression induce Treg (regulatory T cell, Treg) in vitro.
METHODS:placenta-derived mesenchymal stem cells were separated from neonatal placenta; PEGFP - N1 -HLA-G plasmid was transfected in placenta-derived mesenchymal stem cells by liposome transfection.The cells were divided into 3 groups including control group, PEGFP-N1 group and PEGFP-N1-HLA-G group, 5 complex walls in each group. Expression of HLA-G protein was detected by Western Blotting; after identification of cells, healthy human peripheral blood CD4 T lymphocytes were cultured with placenta-derived mesenchymal stem cells with HLA-G positive expression, and the ratio of CD4CD25Foxp3Treg in T lymphocytes was accounted.
RESULTS:After transfection of PEGFP-N1-HLA-G, the placenta-derived mesenchymal stem cells can express HLA-G protein significantly, compared with the control group and PEGFP - N1 group (<0.01). After HLA-G positive placenta-derived mesenchymal stem cells and CD4 + T lymphocytes were cultured for 24 h, the ratio of CD4CD25Foxp3Treg in T lymphocytes was (16.41±0.94)%. After HLA - G positive placenta-derived mesenchymal stem cells and CD4 T lymphocytes were cultured for 48 h, the ratio of CD4CD25Foxp3Treg in T lymphocytes was (16.46±0.59)% significantly, compared with the control group and PEGFP - N1 group (<0.01).
CONCLUSIONS:Placenta-derived mesenchymal stem cells modified by HLA-G gene can effectively induce CD4CD25Foxp3Treg in vitro.
