Development of double-component rapid curing bioadhesive.
10.7507/1001-5515.201805045
- Author:
Juan HUANG
1
;
Haorong LI
2
;
Qian WANG
3
Author Information
1. College of Life Sciences, Zhejiang Sci-Tec University, Hangzhou 310018, P.R.China.
2. Hangzhou Research Institute of Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Hangzhou 310018, P.R.China.
3. Hangzhou Research Institute of Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Hangzhou 310018, P.R.China.QianWang@mail.ipc.ac.cn.
- Publication Type:Journal Article
- Keywords:
adhesive protein;
hyaluronic acid;
layer-by-layer assembly;
mussel foot protein;
rapid solidification
- From:
Journal of Biomedical Engineering
2018;35(6):921-927
- CountryChina
- Language:Chinese
-
Abstract:
Mussel foot proteins (MFp) could cure rapidly under water and adhere to different substrates. It has broad application prospects as an biocompatible bioglue. The soluble recombinant SUMO-MFp fusion protein (SFp3) was efficiently expressed in , and about 5% of tyrosine of SFp3 were converted into DOPA by using mushroom tyrosinase. The adhesion strength of the mixture of DOPA-containing SFp3 (DSFp3) and hyaluronic acid (MW = 1 500 kD) was more than twice that of the cyanoacrylate-based tissue adhesives, Dermabond , and it reached 52% of its maximal strength within 5 minutes on cowhide. A layer-by-layer assembly of hyaluronic acid with DSFp3 was observed to form compact sheet structures through biofilm interferometry assay and scanning electron microscopy. This work provides a solution and theoretical basis for the low adhesion strength and slow curing of protein-based bioglue.
