Effect of down-regulation of sex determining region Y-box 9 on epithelial mesenchymal transition and cloning of oral squamous carcinoma cells.
- VernacularTitle:下调性别决定区Y框蛋白9对口腔鳞癌细胞上皮间质转化及克隆能力的影响
- Author:
Wen-Li YANG
1
;
Ming-Lei SUN
1
;
Peng ZHANG
1
;
Wei-Wei YU
1
;
Hai-Xia ZHOU
1
;
Qiang SUN
1
Author Information
- Publication Type:Journal Article
- Keywords: cell clones; epithelial mesenchymal transition; oral squamous cell carcinoma; sex determining region Y-box 9
- MeSH: Cadherins; Carcinoma, Squamous Cell; Cell Line, Tumor; Cell Movement; Down-Regulation; Epithelial-Mesenchymal Transition; Humans; Mouth Neoplasms; Vimentin
- From: West China Journal of Stomatology 2019;37(1):13-18
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of sex determining region Y-box 9 (SOX9) on epithelial mesenchymal transition (EMT) and cloning of oral squamous cell carcinoma (OSCC).
METHODS:siRNA control, SOX9 siRNA were transfected into BcaCD885 cells in OSCC. Simultaneously, cells that did not undergo transfection were used as the control. Quantitative real time polymerase chain reaction (qRT-PCR) and Western blot were used to select SOX9 siRNA1 with enhanced interference effect. A cell cloning assay was used to determine the cell's clone formation ability. E-cadherin and Vimentin expressions were detected by immunofluorescence. The expressions of E-cadherin, matrix metalloprotease 2 (MMP-2), Vimentin and matrix metalloprotease 9 (MMP-9) were detected by Western blot. Cell invasion and migration were detected in the Transwell compartment.
RESULTS:The levels of SOX9 mRNA and protein in SOX9 siRNA cells were significantly lower than those of the control (P<0.05). An increase in the number of SOX9 siRNA1 cell clonesled to the considerable decrease of the number of cell invasion and migration. In addition, levels of MMP-2 and MMP-9 proteins in cells decreased significantly compared with the control (P<0.05). The level of Vimentin expression in SOX9 siRNA1 cells decreased, and expression level of E-cadherin was elevated. Cell EMT was inhibited compared with the control, and the difference was statistically significant (P<0.05).
CONCLUSIONS:Down-regulation of SOX9 inhibited EMT, clonogenic formation, cell invasion and OSCC migration.
