Impact of Taurine on the proliferation and apoptosis of human cervical carcinoma cells and its mechanism.
10.1097/CM9.0000000000000162
- Author:
Hua LI
1
;
Wen-Jing RUAN
1
;
Li-Qiao LIU
1
;
Hui-Fang WAN
1
;
Xiao-Hong YANG
1
;
Wei-Feng ZHU
1
;
Le-Han YU
1
;
Xia-Li ZHANG
2
;
Fu-Sheng WAN
1
Author Information
1. Department of Biochemistry and Molecular Biology, Medical College of Nanchang University, Nanchang, Jiangxi 330006, China.
2. Experimental Animal Center, Nanchang University, Nanchang, Jiangxi 330006, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Female;
Hepatocyte Growth Factor;
metabolism;
Humans;
Proto-Oncogene Proteins;
metabolism;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Taurine;
drug effects;
pharmacology;
Uterine Cervical Neoplasms;
metabolism;
bcl-2-Associated X Protein;
metabolism
- From:
Chinese Medical Journal
2019;132(8):948-956
- CountryChina
- Language:English
-
Abstract:
BACKGROUND:Cervical cancer has the fourth highest incidence and mortality rate of all cancers in women worldwide; it seriously harms their physical and mental health. The aim of this study was to observe the roles and preliminary mechanism of Taurine (Tau)-induced apoptosis in cervical cancer cells.
METHODS:Cells from the human cervical cancer cell line SiHa were transfected with the recombinant plasmid pEGFP-N1-MST1 (mammalian sterile 20-like kinase 1); then, the cell proliferation activity was analyzed by the MTT assay, cell apoptosis by flow cytometry, and the related protein levels by Western blotting.
RESULTS:Tau inhibited the proliferation of SiHa cells and induced apoptosis in these cells (the apoptotic rate was 21.95% in the Tau 160 mmol/L group and 30% in the Tau 320 mmol/L group), upregulated the expression of the MST1 (control, 0.53; Tau 40-320 mmol/L groups, 0.84-1.45) and Bax (control, 0.45; Tau 40-320 mmol/L groups, 0.64-1.51) proteins (P < 0.01), and downregulated the expression of Bcl-2 (control, 1.28, Tau 40-320 mmol/L groups, 0.93-0.47) (P < 0.01). The overexpression of MST1 promoted the apoptosis of SiHa cells, enhanced the apoptosis-inductive effects of Tau (P < 0.01), upregulated the expression of the proapoptotic proteins p73, p53, PUMA (p53 upregulated modulator of apoptosis), and caspase-3, and promoted the phosphorylation of YAP (Yes-associated protein).
CONCLUSIONS:Tau inhibited the proliferation and induced the apoptosis of cervical cancer SiHa cells. The MST1 protein plays an important role in the Tau-induced apoptosis of cervical cancer cells.
