Establishment of Cs γ ray Combined with Cyclophosphamide and Chloramphenicol-Mediated Mouse Model of Acquired Aplastic Anemia.
10.19746/j.cnki.issn.1009-2137.2019.02.035
- Author:
Le ZHANG
1
;
Xin-Long SONG
2
;
Ai-Di WANG
1
;
Shan ZHANG
1
;
Ping-Ping WANG
1
;
Bao-Shan LIU
3
,
4
Author Information
1. Department of Traditional Chinese Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China.
2. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
3. Department of Traditional Chinese Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China,Email: lbs-cd@
4. com.
- Publication Type:Journal Article
- MeSH:
Anemia, Aplastic;
Animals;
Chloramphenicol;
Cyclophosphamide;
Gamma Rays;
Mice;
Mice, Inbred BALB C
- From:
Journal of Experimental Hematology
2019;27(2):527-533
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To improve and establish the mouse model with aplastic anemia (AA) mediated by Cs γ-ray irradiation combined with cyclophosphamide (CTX) and chloramphenicol (CHL) injection,so as to provide a stable model for studying the pathogenesis and treatment of AA .
METHODS:The BALB/c mice were exposed to Cs γ-ray of 3-5 Gy(91 cGy/min) and were intraperitoneally injected with CTX of 25 mg/(kg.d) and CHL of 62.5 mg/(kg.d) at D 4,5 and 6 after irradiation; the WBC, platelet and reticulocyte counts in peripheral blood as well as the mucleated cell count in bone marrow and bone marrow smears were detected .
RESULTS:The 3-lineage cells in peripheral blood of BALB/c mouse model with acquired AA were rapidly reduced, especially WBC, platelet and reticulocyte counts were lowest at D 14,the 3-lineage cells in peripheral blood were still severely reduced at D 28; the nucleated cell count in bone marrow significantly dcreased,the bone marrow hyperplasia was reduced or severely reduced; the pathological sections of bone marrow showed the severe reduction of hematopoietic cells and the increased of non-hematopoietic cells such as fat cells.
CONCLUSION:The mouse model with acquired AA has been established by Cs γ-ray irradiation combined with CTX and CHL injection. All detection indicators of this model reach to diagnostic criteria for acquired AA,therefore this mouse model may be used as the model for study of pathogenesis and treatment of acquired AA.
