Establishment of Antibody-induced Immune Hemolytic Disease Model in SD Rats and Their Pathotogical Changes.
10.19746/j.cnki.issn.1009-2137.2019.03.040
- Author:
Sai YAN
1
;
Li-Wei LI
1
;
Zhi-Qiang LI
2
,
3
Author Information
1. Department of Blood Transfusion, The Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, China.
2. Department of Blood Transfusion, The Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, China,E-mail: kcb039@
3. com.
- Publication Type:Journal Article
- MeSH:
Animals;
Hemolysis;
Liver;
Mice, Inbred BALB C;
Rats;
Rats, Sprague-Dawley;
Reproducibility of Results
- From:
Journal of Experimental Hematology
2019;27(3):887-892
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the model of antibody-induced immune hemolytic disease in SD rats so as to provide an experimental platform for the exploration of its pathogenesis, course of disease and evaluation of drug efficacy.
METHODS:The red blood cells(RBC) of SD rats were isolated and intraperitoneally injected into BALB/c mice to induce production of the antiserum to SD rat RBC. Twenty SD rats were randomly divided into 2 groups. The rats in the model group were injected with 0.1 ml antiserum via tail vein; the rats in the control group were injected with 0.1 ml saline via tail vein.The symptoms of rats, hemolysis-related indexes and histopathological changes of the main organs were observed in both groups after injection.
RESULTS:After the injection of antiserum, the SD rats in the model group displayed nasal flaring, laziness, decrease of ingestion and water intake, skin and mucosal jaundice, and gross hemoglobinuria. At the 4th day after the injection, the body weight of SD rats in the model group was significantly lower than that in the control group (P＜0.01), and the coefficiens of liver and spleen increased significantly (P＜0.01); The levels of WBC, MCV, MCH, DBIL, DBIL/TBIL and FHb all increased statistically significantly, and RBC, Hb, HCT, MCHC and Plt levels decreased significantly in comparison with the control group (P＜0.01). In the SD rats of model group, the hemolytic pathological changes were observed in liver, spleen, kidney, lung and small intestine, and erythroid proliferation was observed in bone marrow smears.
CONCLUSION:The immune hemolytic disease model of SD rats can be successfully established by injecting the serum aginst SD rat red blood cells into the tail vein of SD rats, showing the high success rate, good reproducibility and low cost.
