Dynamics of Proliferation and Differentiation after Transplantation of Hematopoietic Cells in Mouse.
10.19746/j.cnki.issn.1009-2137.2019.03.052
- Author:
Jin-Fang LIU
1
;
Ke ZHAO
2
;
Hui-Ying GAO
2
;
Rui GAO
2
;
Yi-Qun ZHAN
2
;
Xiao-Ming YANG
3
;
Chang-Yan LI
4
,
5
;
Kai-Hua WEI
5
,
6
Author Information
1. Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, China,Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China,Institute of Lifeomics, Academy of Military Medical Sciences, Beijing 102206, China.
2. Institute of Lifeomics, Academy of Military Medical Sciences, Beijing 102206, China.
3. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institute of Lifeomics, Academy of Military Medical Sciences, Beijing 102206, China.
4. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institute of Lifeomics, Academy of Military Medical Sciences, Beijing 102206, China,E-mail: fmmli@
5. com.
6. Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, China;Institute of Lifeomics, Academy of Military Medical Sciences, Beijing 102206, China,,E-mail: wkh2006@
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow;
Bone Marrow Cells;
Cell Differentiation;
Cell Proliferation;
Hematopoietic Stem Cell Transplantation;
Hematopoietic Stem Cells;
Mice;
Mice, Inbred C57BL
- From:
Journal of Experimental Hematology
2019;27(3):958-963
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of Listeria monocytogenes infection on hematopoietic stem and progenitor cell (HSPC) composition, cell cycle and cell colony-forming ability in mouse bone marrow.
METHODS:The C57BL/6J mice were divided into infected group and control group. The mice in injected group were infected intraperitoneally with 6.7×10 CFU Listeria monocytogenes,while the mice in control group were injecfed with PBS of same volume.The serum levels of IFNγ were detected at different time points. After 24 hours, the HS/PC composition, cell cycle and cell colony-forming ability in bone marrow of mice were measured, and the difference between the control group and the infected group was statistically analyzed.
RESULTS:Serum IFNγ levels peaked at 24 hours after infection with Listeria monocytogenes. After 24 h, the proportion of LSK, LSK in S phase, and short-term hematopoietic stem cells (ST-HSC) in the infected group were significantly higher than those in the control group (P＜0.001), long-term hematopoietic stem cells (LT-HSC) and the proportion of LT-HSC in S phase were significantly increased (P＜0.01), and the cell colony-forming ability of bone marrow significantly decreased (P＜0.01). [WTHZ]Conclusion: [WTB1]After infection with Listeria monocytogenes, bone marrow hematopoietic stem cells enter the proliferative state from rest, the cell colony-forming ability decreases, suggesting that Listeria monocytogenes infection can cause hematopoietic stem cell depletion.
