Effect of Inhibiting NCL Expression on Drug-Resistance of Chronic Myeloid Leukemia Cell Line K562 and Its Resistant Cells.
10.19746/j.cnki.issn.1009-2137.2019.04.012
- Author:
Min-Hui LIN
1
;
Yan-Xin CHEN
1
;
Yun ZHANG
1
;
Zheng-Jun WU
1
;
Wei-Juan LIU
1
;
Jian-Da HU
2
,
3
Author Information
1. Fujian Medical University Union Hospital, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fuzhou 350001, Fujian Province, China.
2. Fujian Medical University Union Hospital, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fuzhou 350001, Fujian Province, China,E-mail: drjiandahu@
3. com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Doxorubicin;
Drug Resistance, Neoplasm;
Humans;
K562 Cells;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
Phosphoproteins;
RNA-Binding Proteins
- From:
Journal of Experimental Hematology
2019;27(4):1064-1070
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct a K562 and adriamycin-resistant K562 (KAR) cell line with stably down-regulation of NCL (nucleolin) expression, and to investigate the effect of NCL down-regulation on the drug resistance in K562 and KAR cells.
METHODS:K562 and KAR cells were infected with lentivirus, and stably transfected cell clones were obtained by puromycin screening. The cell proliferation was detected by MTS assay, the cell apoptosis was detected by flow cytometry, and the expression level of drug resistance related genes was detected by real-time PCR.
RESULTS:The K562 and KAR cells with stable down-regulation of NCL were successfully constructed. Compared with the control group, the proliferation of K562 and KAR cells with down-regulating NCL expression decreased significantly (P ＜0.05), the apoptosis of cells increased significantly (P ＜0.05), and cell resistance to adriamycin was down-regulated.
CONCLUSION:Inhibition of NCL expression may increase the sensitivity of cells to adriamycin, which may be related with the promotion of apoptosis of K562 and KAR cells.
